DEPOD - human DEPhOsphorylation Database

Basic Information
Phosphatases
Pathway
Interacting Proteins
Phosphorylating Kinases

Gene Name	WAS (QuickGO)	Interactive visualization of WAS structures (A quick tutorial to explore the interctive visulaization) Representative structure: 2OT0
Synonyms	WAS, IMD2
Protein Name	WAS
Alternative Name(s)	Wiskott-Aldrich syndrome protein;WASp;
Protein Family	noSimilarity_annotations
EntrezGene ID	7454 (Comparitive Toxicogenomics)
UniProt AC (Human)	P42768 (protein sequence)
Enzyme Class	N/A
Molecular Weight	52913 Dalton
Protein Length	502 amino acids (AA)
Genome Browsers	NCBI \| ENSG00000015285 (Ensembl) \| UCSC
Crosslinking annotations	Query our ID-mapping table
Orthologues	Quest For Orthologues (QFO) \| GeneTree \| eggNOG - KOG3671 \| eggNOG - ENOG4111M1J
Phosphorylation Network	Visualize

Domain organization, Expression, Diseases

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Localization, Function, Catalytic activity and Sequence

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Motif information from Eukaryotic Linear Motif atlas (ELM)

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Gene Ontology (P: Process; F: Function and C: Component terms)

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KEGG Pathway
Reactome Pathway

Pathway ID	Pathway Name	Pathway Description (KEGG)
hsa04062	Chemokine signaling pathway	Inflammatory immune response requires the recruitment of leukocytes to the site of inflammation upon foreign insult. Chemokines are small chemoattractant peptides that provide directional cues for the cell trafficking and thus are vital for protective host response. In addition, chemokines regulate plethora of biological processes of hematopoietic cells to lead cellular activation, differentiation and survival.The chemokine signal is transduced by chemokine receptors (G-protein coupled receptors) expressed on the immune cells. After receptor activation, the alpha- and beta-gamma-subunits of G protein dissociate to activate diverse downstream pathways resulting in cellular polarization and actin reorganization. Various members of small GTPases are involved in this process. Induction of nitric oxide and production of reactive oxygen species are as well regulated by chemokine signal via calcium mobilization and diacylglycerol production.
hsa04144	Endocytosis	Endocytosis is a mechanism for cells to remove ligands, nutrients, and plasma membrane (PM) proteins, and lipids from the cell surface, bringing them into the cell interior. Transmembrane proteins entering through clathrin-dependent endocytosis (CDE) have sequences in their cytoplasmic domains that bind to the APs (adaptor-related protein complexes) and enable their rapid removal from the PM. In addition to APs and clathrin, there are numerous accessory proteins including dynamin. Depending on the various proteins that enter the endosome membrane, these cargoes are sorted to distinct destinations. Some cargoes, such as nutrient receptors, are recycled back to the PM. Ubiquitylated membrane proteins, such as activated growth-factor receptors, are sorted into intraluminal vesicles and eventually end up in the lysosome lumen via multivesicular endosomes (MVEs). There are distinct mechanisms of clathrin-independent endocytosis (CIE) depending upon the cargo and the cell type.
hsa04520	Adherens junction	Cell-cell adherens junctions (AJs), the most common type of intercellular adhesions, are important for maintaining tissue architecture and cell polarity and can limit cell movement and proliferation. At AJs, E-cadherin serves as an essential cell adhesion molecules (CAMs). The cytoplasmic tail binds beta-catenin, which in turn binds alpha-catenin. Alpha-catenin is associated with F-actin bundles directly and indirectly. The integrity of the cadherin-catenin complex is negatively regulated by phosphorylation of beta-catenin by receptor tyrosine kinases (RTKs) and cytoplasmic tyrosine kinases (Fer, Fyn, Yes, and Src), which leads to dissociation of the cadherin-catenin complex. Integrity of this complex is positively regulated by beta -catenin phosphorylation by casein kinase II, and dephosphorylation by protein tyrosine phosphatases. Changes in the phosphorylation state of beta-catenin affect cell-cell adhesion, cell migration and the level of signaling beta-catenin. Wnt signaling acts as a positive regulator of beta-catenin by inhibiting beta-catenin degradation, which stabilizes beta-catenin, and causes its accumulation. Cadherin may acts as a negative regulator of signaling beta-catenin as it binds beta-catenin at the cell surface and thereby sequesters it from the nucleus. Nectins also function as CAMs at AJs, but are more highly concentrated at AJs than E-cadherin. Nectins transduce signals through Cdc42 and Rac, which reorganize the actin cytoskeleton, regulate the formation of AJs, and strengthen cell-cell adhesion.
hsa04530	Tight junction	Tight junctions (TJs) are essential for establishing a selectively permeable barrier to diffusion through the paracellular space between neighboring cells. TJs are composed of at least three types of transmembrane protein -occludin, claudin and junctional adhesion molecules (JAMs)- and a cytoplasmic 'plaque' consisting of many different proteins that form large complexes. These are proposed to be involved in junction assembly, barrier regulation, cell polarity, gene transcription, and other pathways.
hsa04666	Fc gamma R-mediated phagocytosis	Phagocytosis plays an essential role in host-defense mechanisms through the uptake and destruction of infectious pathogens. Specialized cell types including macrophages, neutrophils, and monocytes take part in this process in higher organisms. After opsonization with antibodies (IgG), foreign extracellular materials are recognized by Fc gamma receptors. Cross-linking of Fc gamma receptors initiates a variety of signals mediated by tyrosine phosphorylation of multiple proteins, which lead through the actin cytoskeleton rearrangements and membrane remodeling to the formation of phagosomes. Nascent phagosomes undergo a process of maturation that involves fusion with lysosomes. The acquisition of lysosomal proteases and release of reactive oxygen species are crucial for digestion of engulfed materials in phagosomes.
hsa04810	Regulation of actin cytoskeleton
hsa05100	Bacterial invasion of epithelial cells	Many pathogenic bacteria can invade phagocytic and non-phagocytic cells and colonize them intracellularly, then become disseminated to other cells. Invasive bacteria induce their own uptake by non-phagocytic host cells (e.g. epithelial cells) using two mechanisms referred to as zipper model and trigger model. Listeria, Staphylococcus, Streptococcus, and Yersinia are examples of bacteria that enter using the zipper model. These bacteria express proteins on their surfaces that interact with cellular receptors, initiating signalling cascades that result in close apposition of the cellular membrane around the entering bacteria. Shigella and Salmonella are the examples of bacteria entering cells using the trigger model. These bacteria use type III secretion systems to inject protein effectors that interact with the actin cytoskeleton.
hsa05130	Pathogenic Escherichia coli infection	Enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) are closely related pathogenic strains of Escherichia coli. The hallmark of EPEC/EHEC infections [DS:H00278 H00277] is induction of attaching and effacing (A/E) lesions that damage intestinal epithelial cells. The capacity to form A/E lesions is encoded mainly by the locus of enterocyte effacement (LEE) pathogenicity island. Tir, Map, EspF, EspG are known LEE-encoded effector proteins secreted via the type III secretion system, which is also LEE-encoded, into the host cell. EPEC and EHEC Tir's link the extracellular bacterium to the cell cytoskeleton. Map and EspF are involved in mitochondrion membrane permeabilization. EspG interacts with tubulins and stimulates microtubule destabilization. LEE-encoded adhesin or intimin (Eae) is exported via the general secretory pathway to the periplasm, where it is inserted into the outer membrane. In addition to Tir, two potential host cell-carried intimin receptors, beta1 integrin (ITGB1) and nucleolin (NCL), have so far been identified. The distinguishing feature of EHEC is the elaboration of Shiga-like toxin (Stx). Stx cleaves ribosomal RNA, thereby disrupting protein synthesis and killing the intoxicated epithelial or endothelial cells.
hsa05131	Shigellosis	Shigellosis, or bacillary dysentery, is an intestinal infection caused by Shigella, a genus of enterobacteria. Shigella are potential food-borne pathogens that are capable of colonizing the intestinal epithelium by exploiting epithelial-cell functions and circumventing the host innate immune response. During basolateral entry into the host-cell cytoplasm, Shigella deliver a subset of effectors into the host cells through the type III secretion system. The effectors induce membrane ruffling through the stimulation of the Rac1-WAVE-Arp2/3 pathway, enabling bacterial entry into the epithelial cells. During multiplication within the cells, Shigella secrete another subset of effectors. VirG induces actin polymerization at one pole of the bacteria, allowing the bacteria to spread intracellularly and to infect adjacent cells. OspF, OspG and IpaH(9.8) downregulate the production of proinflammatory cytokines such as IL-8, helping bacteria circumvent the innate immune response.
hsa05132	Salmonella infection	Salmonella infection usually presents as a self-limiting gastroenteritis or the more severe typhoid fever and bacteremia. The common disease-causing Salmonella species in human is a single species, Salmonella enterica, which has numerous serovars.Following intestinal colonization Salmonella inject effector proteins into the host cells using a type III secretion system (T3SS), T3SS1. Then a small group of effector proteins induce rearrangement of the actin cytoskeleton resulting in membrane ruffles and rapid internalization of the bacteria.The T3SS2 is responsible for translocating effector proteins that direct Salmonella-containing vacuole (SCV) maturation. The majority of the bacteria are known to survive and replicate in SCV.
hsa05231	Choline metabolism in cancer	Abnormal choline metabolism is emerging as a metabolic hallmark that is associated with oncogenesis and tumour progression. Following transformation, oncogenic signalling via pathways such as the RAS and PI3K-AKT pathways, and transcription factors associated with oncogenesis such as hypoxia-inducible factor 1 (HIF1) mediate overexpression and activation of choline cycle enzymes, which causes increased levels of choline-containing precursors and breakdown products of membrane phospholipids. These products of choline phospholipid metabolism, such as phosphocholine (PCho), diacylglycerol (DAG) and phosphatidic acid, may function as second messengers that are essential for the mitogenic activity of growth factors, particularly in the activation of the ras-raf-1-MAPK cascade and protein kinase C pathway.

Pathway ID	Pathway Name	Pathway Description (KEGG)
R-HSA-202433	Generation of second messenger molecules.	In addition to serving as a scaffold via auto-phosphorylation, ZAP-70 also phosphorylates a restricted set of substrates following TCR stimulation - including LAT and SLP-76. These substrates have been recognized to play pivotal role in TCR signaling by releasing second messengers. When phosphorylated, LAT and SLP-76 act as adaptor proteins which serve as nucleation points for the construction of a higher order signalosome: GADS, PLC-gamma1 and GRB2 bind to the LAT on the phosphorylated tyrosine residues (steps 8 and 13). SLP-76 and SOS are then moved to the signalosome by interacting with the SH3 domains of GRB2 and GADS via their proline rich sequences (step 9). Three SLP-76 acidic domain N-term tyrosine residues are phosphorylated by ZAP-70, once SLP-76 binds to GADS (step 10). These phospho-tyrosine residues act as binding sites to the SH2 domains of PLC-gamma1, Vav and Itk (steps 11 and 12). PLC-gamma1 is activated by dual phosphorylation on the tyrosine residues at positions 771, 783 and 1254 by Itk and ZAP-70 (step 14). Phosphorylated PLC-gamma1 subsequently detaches from LAT and SLP-76 and translocates to the plasma membrane by binding to phosphatidylinositol-4,5-bisphosphate (PIP2) via its PH domain (step 15). PLC-gamma1 goes on to hydrolyse PIP2 to second messengers DAG and IP3. These second messengers are involved in PKC and NF-kB activation and calcium mobilization (step 16)
R-HSA-2029482	Regulation of actin dynamics for phagocytic cup formation.	The actin cytoskeleton is fundamental for phagocytosis and members of the Rho family GTPases RAC and CDC42 are involved in actin cytoskeletal regulation leading to pseudopod extension. Active RAC and CDC42 exert their action through the members of WASP family proteins (WASP/N-WASP/WAVE) and ARP2/3 complex. Actin filaments move from the bottom toward the top of the phagocytic cup during pseudopod extension
R-HSA-5663213	RHO GTPases Activate WASPs and WAVEs.	WASP and WAVE proteins belong to the Wiskott-Aldrich Syndrome protein family, with recessive mutations in the founding member WASP being responsible for the X-linked recessive immunodeficieny known as the Wiskott-Aldrich Syndrome. WASP proteins include WASP and WASL (N-WASP). WAVE proteins include WASF1 (WAVE1), WASF2 (WAVE2) and WASF3 (WAVE3). WASPs and WAVEs contain a VCA domain (consisting of WH2 and CA subdomains) at the C-terminus, responsible for binding to G-actin (WH2 subdomain) and the actin-associated ARP2/3 complex (CA subdomain). WASPs contain a WH1 (WASP homology 1) domain at the N-terminus, responsible for binding to WIPs (WASP-interacting proteins). A RHO GTPase binding domain (GBD) is located in the N-terminal half of WASPs and C-terminally located in WAVEs. RHO GTPases activate WASPs by disrupting the autoinhibitory interaction between the GBD and VCA domains, which allows WASPs to bind actin and the ARP2/3 complex and act as nucleation promoting factors in actin polymerization. WAVEs have the WAVE/SCAR homology domain (WHD/SHD) at the N-terminus, which binds ABI, NCKAP1, CYFIP2 and BRK1 to form the WAVE regulatory complex (WRC). Binding of the RAC1:GTP to the GBD of WAVEs most likely induces a conformational change in the WRC that allows activating phosphorylation of WAVEs by ABL1, thus enabling them to function as nucleation promoting factors in actin polymerization through binding G-actin and the ARP2/3 complex (Reviewed by Lane et al. 2014)

BioGRID
IntAct
MINT
ALL

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Interacting partner	Detection method	Interaction type	PubMed IDs	Interaction Score	Source
APBB1	Reconstituted Complex	physical	16055720, (Europe PMC)	NA	BioGRID
APPBP2	Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical, physical association	25416956, (Europe PMC)	0.56	BioGRID, IntAct
ARPC2	Reconstituted Complex	physical	11943145, (Europe PMC)	NA	BioGRID
ARPC3	Affinity Capture-MS, Reconstituted Complex, Two-hybrid, pull down	association, physical	12769847, 23148219, 9889097, (Europe PMC)	0.35	BioGRID, IntAct
ARPC4	Affinity Capture-MS, pull down	association, physical	12769847, (Europe PMC)	0.35	BioGRID, IntAct
BAIAP2L1	Reconstituted Complex	physical	22921828, (Europe PMC)	NA	BioGRID
BTK	Affinity Capture-Western, Reconstituted Complex, pull down	physical, physical association	10427990, 8892607, (Europe PMC)	0.40	BioGRID, IntAct
CBL	Affinity Capture-Western	physical	23684068, (Europe PMC)	NA	BioGRID
CBLB	Affinity Capture-Western	physical	23684068, (Europe PMC)	NA	BioGRID
CDC42	Affinity Capture-Western, Reconstituted Complex, Two-hybrid, circular dichroism, classical fluorescence spectroscopy, far western blotting, imaging technique, nuclear magnetic resonance, pull down, surface plasmon resonance, two hybrid pooling approach	direct interaction, physical, physical association	10360578, 10360579, 10684602, 10713100, 10724160, 15075243, 15361624, 20936779, 28539360, 8625410, 8643625, 9422512, 9660763, (Europe PMC)	0.44, 0.89	BioGRID, IntAct, MINT
CRKL	Affinity Capture-Western, Reconstituted Complex	physical	11313252, (Europe PMC)	NA	BioGRID
CSNK2A1	Biochemical Activity, protein kinase assay	phosphorylation reaction, physical	12769847, (Europe PMC)	0.44	BioGRID, IntAct
DVL2	Two-hybrid, two hybrid	physical, physical association	21516116, (Europe PMC)	0.37	BioGRID, IntAct, MINT
EGFR	Affinity Capture-Western	physical	9307968, (Europe PMC)	NA	BioGRID
FCHSD2	Two-hybrid, two hybrid	physical, physical association	21988832, (Europe PMC)	0.37	BioGRID, IntAct
FGR	Affinity Capture-Western, Reconstituted Complex, pull down	direct interaction, physical	7565724, 8805332, 8824280, (Europe PMC)	0.44	BioGRID, IntAct, MINT
FNBP1	Affinity Capture-MS, Affinity Capture-Western	physical	16418535, 19041431, (Europe PMC)	NA	BioGRID
FYB1	Affinity Capture-Western	physical	10747096, (Europe PMC)	NA	BioGRID
FYN	Affinity Capture-Western, Biochemical Activity, Reconstituted Complex, anti bait coimmunoprecipitation, experimental interaction detection, pull down	direct interaction, phosphorylation reaction, physical, physical association	10532312, 12431372, 14707117, 7565724, 8805332, (Europe PMC)	0.70	BioGRID, IntAct, MINT
GAS7	Affinity Capture-Western, Reconstituted Complex, pull down	physical, physical association	16055720, 26506240, (Europe PMC)	0.40	BioGRID, IntAct, MINT
GRB2	Affinity Capture-Western, Reconstituted Complex, pull down	physical, physical association	8805332, 8892607, 9307968, (Europe PMC)	0.40	BioGRID, IntAct
HCK	Reconstituted Complex, anti bait coimmunoprecipitation, far western blotting, pull down	association, physical, physical association	12029088, 19234535, (Europe PMC)	0.72	BioGRID, IntAct, MINT
ITK	Reconstituted Complex, pull down	physical, physical association	8810341, 8892607, (Europe PMC)	0.40	BioGRID, IntAct
ITSN1	Affinity Capture-Western	physical	15824104, 18539136, (Europe PMC)	NA	BioGRID
ITSN2	Affinity Capture-Western, Reconstituted Complex, Two-hybrid	physical	11748279, 22558309, (Europe PMC)	NA	BioGRID
JMJD6	Affinity Capture-Western	physical	20574068, (Europe PMC)	NA	BioGRID
KDM4A	Affinity Capture-Western	physical	20574068, (Europe PMC)	NA	BioGRID
KDM6B	Affinity Capture-Western	physical	20574068, (Europe PMC)	NA	BioGRID
LCK	Affinity Capture-Western, Biochemical Activity, pull down	physical, physical association	16107303, 16293614, (Europe PMC)	0.40	BioGRID, IntAct, MINT
LCP2	Affinity Capture-Western	physical	10747096, (Europe PMC)	NA	BioGRID
LRPPRC	Affinity Capture-MS, anti tag coimmunoprecipitation	association, physical	26496610, (Europe PMC)	0.35	BioGRID, IntAct
NCK1	Affinity Capture-Western, Reconstituted Complex, coimmunoprecipitation, imaging technique, pull down	colocalization, direct interaction, physical, physical association	10747096, 12135674, 24287595, 7565724, (Europe PMC)	0.57	BioGRID, IntAct, MINT
NCK2	Affinity Capture-Western, Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical, physical association	24287595, 25416956, (Europe PMC)	0.56	BioGRID, IntAct
PACSIN1	Affinity Capture-MS	physical	19041431, (Europe PMC)	NA	BioGRID
PACSIN2	Affinity Capture-MS	physical	19041431, (Europe PMC)	NA	BioGRID
PACSIN3	Affinity Capture-MS	physical	19041431, (Europe PMC)	NA	BioGRID
PIK3R1	Affinity Capture-Western, Reconstituted Complex	physical	8805332, (Europe PMC)	NA	BioGRID
PLCG1	Reconstituted Complex, pull down	physical, physical association	8805332, 8824280, 8892607, (Europe PMC)	0.40	BioGRID, IntAct
PRPF40A	Reconstituted Complex	physical	16055720, (Europe PMC)	NA	BioGRID
PSTPIP1	Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex, anti bait coimmunoprecipitation, imaging technique	colocalization, physical, physical association	14707117, 19041431, 9488710, (Europe PMC)	0.46	BioGRID, IntAct, MINT
RAC1	Reconstituted Complex	physical	8643625, (Europe PMC)	NA	BioGRID
RANBP2	Affinity Capture-MS, Affinity Capture-Western, Biochemical Activity	physical	26261240, (Europe PMC)	NA	BioGRID
RBBP5	Affinity Capture-Western	physical	20574068, (Europe PMC)	NA	BioGRID
RELA	Affinity Capture-Western	physical	26261240, (Europe PMC)	NA	BioGRID
RGS14	Two-hybrid, two hybrid	physical, physical association	21988832, (Europe PMC)	0.37	BioGRID, IntAct
RHOJ	Two-hybrid, pull down	physical, physical association	10967094, 14521508, (Europe PMC)	0.40	BioGRID, IntAct
SAE1	Affinity Capture-MS	physical	26261240, (Europe PMC)	NA	BioGRID
SORBS2	Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical, physical association	25416956, (Europe PMC)	0.56	BioGRID, IntAct
SRC	Affinity Capture-Western, Reconstituted Complex	physical	8805332, 8824280, (Europe PMC)	NA	BioGRID
SRGAP2	Affinity Capture-MS	physical	19041431, (Europe PMC)	NA	BioGRID
TCERG1	Reconstituted Complex	physical	16055720, (Europe PMC)	NA	BioGRID
TEC	Reconstituted Complex, pull down	physical, physical association	8892607, (Europe PMC)	0.40	BioGRID, IntAct
TNK2	Affinity Capture-Western, Biochemical Activity	physical	16257963, (Europe PMC)	NA	BioGRID
TRIP10	Affinity Capture-Western, Reconstituted Complex, Two-hybrid	physical	10713100, 21516116, (Europe PMC)	NA	BioGRID
UBA2	Affinity Capture-MS, Affinity Capture-Western	physical	26261240, (Europe PMC)	NA	BioGRID
UBE2I	Affinity Capture-MS, Affinity Capture-Western	physical	26261240, (Europe PMC)	NA	BioGRID
VASP	Reconstituted Complex	physical	11598004, (Europe PMC)	NA	BioGRID
WBP4	Reconstituted Complex, pull down	physical, physical association	16055720, (Europe PMC)	0.40	BioGRID, IntAct, MINT
WIPF1	Affinity Capture-Western, Reconstituted Complex, Two-hybrid, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, pull down, two hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical, physical association	10202051, 16488394, 17213309, 19805221, 21988832, 25416956, 9405671, 9694849, (Europe PMC)	0.40, 0.49, 0.95	BioGRID, IntAct
WIPF2	Affinity Capture-Western, Two-hybrid, two hybrid	physical, physical association	12213210, 21988832, (Europe PMC)	0.37	BioGRID, IntAct

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Interacting partner	Detection method	Interaction type	PubMed IDs	Interaction Score	Source
ACTR2	pull down	association	12769847, (Europe PMC)	0.35	IntAct
ACTR3	pull down	association	12769847, (Europe PMC)	0.35	IntAct
APPBP2	Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical, physical association	25416956, (Europe PMC)	0.56	BioGRID, IntAct
ARPC1B	fluorescence microscopy, pull down	colocalization, direct interaction, physical association	10861055, 11943145, (Europe PMC)	0.64	IntAct, MINT
ARPC3	Affinity Capture-MS, Reconstituted Complex, Two-hybrid, pull down	association, physical	12769847, 23148219, 9889097, (Europe PMC)	0.35	BioGRID, IntAct
ARPC4	Affinity Capture-MS, pull down	association, physical	12769847, (Europe PMC)	0.35	BioGRID, IntAct
BTK	Affinity Capture-Western, Reconstituted Complex, pull down	physical, physical association	10427990, 8892607, (Europe PMC)	0.40	BioGRID, IntAct
CDC42	Affinity Capture-Western, Reconstituted Complex, Two-hybrid, circular dichroism, classical fluorescence spectroscopy, far western blotting, imaging technique, nuclear magnetic resonance, pull down, surface plasmon resonance, two hybrid pooling approach	direct interaction, physical, physical association	10360578, 10360579, 10684602, 10713100, 10724160, 15075243, 15361624, 20936779, 28539360, 8625410, 8643625, 9422512, 9660763, (Europe PMC)	0.44, 0.89	BioGRID, IntAct, MINT
CSNK2A1	Biochemical Activity, protein kinase assay	phosphorylation reaction, physical	12769847, (Europe PMC)	0.44	BioGRID, IntAct
CTTN	anti bait coimmunoprecipitation, fluorescence microscopy	colocalization, physical association	15169891, (Europe PMC)	0.46	IntAct
DVL2	Two-hybrid, two hybrid	physical, physical association	21516116, (Europe PMC)	0.37	BioGRID, IntAct, MINT
FCHSD2	Two-hybrid, two hybrid	physical, physical association	21988832, (Europe PMC)	0.37	BioGRID, IntAct
FGR	Affinity Capture-Western, Reconstituted Complex, pull down	direct interaction, physical	7565724, 8805332, 8824280, (Europe PMC)	0.44	BioGRID, IntAct, MINT
FYN	Affinity Capture-Western, Biochemical Activity, Reconstituted Complex, anti bait coimmunoprecipitation, experimental interaction detection, pull down	direct interaction, phosphorylation reaction, physical, physical association	10532312, 12431372, 14707117, 7565724, 8805332, (Europe PMC)	0.70	BioGRID, IntAct, MINT
GAS7	Affinity Capture-Western, Reconstituted Complex, pull down	physical, physical association	16055720, 26506240, (Europe PMC)	0.40	BioGRID, IntAct, MINT
GRB2	Affinity Capture-Western, Reconstituted Complex, pull down	physical, physical association	8805332, 8892607, 9307968, (Europe PMC)	0.40	BioGRID, IntAct
HCK	Reconstituted Complex, anti bait coimmunoprecipitation, far western blotting, pull down	association, physical, physical association	12029088, 19234535, (Europe PMC)	0.72	BioGRID, IntAct, MINT
HCLS1	pull down	physical association	21398607, (Europe PMC)	0.40	IntAct
ITK	Reconstituted Complex, pull down	physical, physical association	8810341, 8892607, (Europe PMC)	0.40	BioGRID, IntAct
LCK	Affinity Capture-Western, Biochemical Activity, pull down	physical, physical association	16107303, 16293614, (Europe PMC)	0.40	BioGRID, IntAct, MINT
LRPPRC	Affinity Capture-MS, anti tag coimmunoprecipitation	association, physical	26496610, (Europe PMC)	0.35	BioGRID, IntAct
NCK1	Affinity Capture-Western, Reconstituted Complex, coimmunoprecipitation, imaging technique, pull down	colocalization, direct interaction, physical, physical association	10747096, 12135674, 24287595, 7565724, (Europe PMC)	0.57	BioGRID, IntAct, MINT
NCK2	Affinity Capture-Western, Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical, physical association	24287595, 25416956, (Europe PMC)	0.56	BioGRID, IntAct
PLCG1	Reconstituted Complex, pull down	physical, physical association	8805332, 8824280, 8892607, (Europe PMC)	0.40	BioGRID, IntAct
PSTPIP1	Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex, anti bait coimmunoprecipitation, imaging technique	colocalization, physical, physical association	14707117, 19041431, 9488710, (Europe PMC)	0.46	BioGRID, IntAct, MINT
PTPN12	anti bait coimmunoprecipitation, phosphatase assay	dephosphorylation reaction, physical association	14707117, 19167335, (Europe PMC)	0.61	IntAct, MINT
PTPN22	phosphatase assay	dephosphorylation reaction	19167335, (Europe PMC)	0.44	IntAct
PTPRB	phosphatase assay	dephosphorylation reaction	19167335, (Europe PMC)	0.44	IntAct
PTPRC	phosphatase assay	dephosphorylation reaction	19167335, (Europe PMC)	0.44	IntAct
PTPRG	phosphatase assay	dephosphorylation reaction	19167335, (Europe PMC)	0.44	IntAct
PTPRJ	phosphatase assay	dephosphorylation reaction	19167335, (Europe PMC)	0.44	IntAct
PTPRO	phosphatase assay	dephosphorylation reaction	19167335, (Europe PMC)	0.44	IntAct
PTPRZ1	phosphatase assay	dephosphorylation reaction	19167335, (Europe PMC)	0.44	IntAct
RAC2	pull down	physical association	14521508, (Europe PMC)	0.40	IntAct
RAC3	pull down	physical association	14521508, (Europe PMC)	0.40	IntAct
RGS14	Two-hybrid, two hybrid	physical, physical association	21988832, (Europe PMC)	0.37	BioGRID, IntAct
RHOJ	Two-hybrid, pull down	physical, physical association	10967094, 14521508, (Europe PMC)	0.40	BioGRID, IntAct
RHOQ	pull down	physical association	14521508, (Europe PMC)	0.40	IntAct
ROBO4	two hybrid	physical association	18948384, (Europe PMC)	0.37	IntAct
SNX33	anti tag coimmunoprecipitation	physical association	19487689, (Europe PMC)	0.40	IntAct, MINT
SNX9	anti tag coimmunoprecipitation, two hybrid	physical association	17242350, 19487689, (Europe PMC)	0.57	IntAct, MINT
SORBS2	Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical, physical association	25416956, (Europe PMC)	0.56	BioGRID, IntAct
TEC	Reconstituted Complex, pull down	physical, physical association	8892607, (Europe PMC)	0.40	BioGRID, IntAct
WAS	classical fluorescence spectroscopy, pull down	direct interaction, physical association	10724160, 12769847, 18650809, (Europe PMC)	0.75	IntAct
WBP4	Reconstituted Complex, pull down	physical, physical association	16055720, (Europe PMC)	0.40	BioGRID, IntAct, MINT
WIPF1	Affinity Capture-Western, Reconstituted Complex, Two-hybrid, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, pull down, two hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical, physical association	10202051, 16488394, 17213309, 19805221, 21988832, 25416956, 9405671, 9694849, (Europe PMC)	0.40, 0.49, 0.95	BioGRID, IntAct
WIPF2	Affinity Capture-Western, Two-hybrid, two hybrid	physical, physical association	12213210, 21988832, (Europe PMC)	0.37	BioGRID, IntAct

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Interacting partner	Detection method	Interaction type	PubMed IDs	Interaction Score	Source
ARPC1B	fluorescence microscopy, pull down	colocalization, direct interaction, physical association	10861055, 11943145, (Europe PMC)	0.64	IntAct, MINT
CDC42	Affinity Capture-Western, Reconstituted Complex, Two-hybrid, circular dichroism, classical fluorescence spectroscopy, far western blotting, imaging technique, nuclear magnetic resonance, pull down, surface plasmon resonance, two hybrid pooling approach	direct interaction, physical, physical association	10360578, 10360579, 10684602, 10713100, 10724160, 15075243, 15361624, 20936779, 28539360, 8625410, 8643625, 9422512, 9660763, (Europe PMC)	0.44, 0.89	BioGRID, IntAct, MINT
DVL2	Two-hybrid, two hybrid	physical, physical association	21516116, (Europe PMC)	0.37	BioGRID, IntAct, MINT
FGR	Affinity Capture-Western, Reconstituted Complex, pull down	direct interaction, physical	7565724, 8805332, 8824280, (Europe PMC)	0.44	BioGRID, IntAct, MINT
FYN	Affinity Capture-Western, Biochemical Activity, Reconstituted Complex, anti bait coimmunoprecipitation, experimental interaction detection, pull down	direct interaction, phosphorylation reaction, physical, physical association	10532312, 12431372, 14707117, 7565724, 8805332, (Europe PMC)	0.70	BioGRID, IntAct, MINT
GAS7	Affinity Capture-Western, Reconstituted Complex, pull down	physical, physical association	16055720, 26506240, (Europe PMC)	0.40	BioGRID, IntAct, MINT
HCK	Reconstituted Complex, anti bait coimmunoprecipitation, far western blotting, pull down	association, physical, physical association	12029088, 19234535, (Europe PMC)	0.72	BioGRID, IntAct, MINT
LCK	Affinity Capture-Western, Biochemical Activity, pull down	physical, physical association	16107303, 16293614, (Europe PMC)	0.40	BioGRID, IntAct, MINT
NCK1	Affinity Capture-Western, Reconstituted Complex, coimmunoprecipitation, imaging technique, pull down	colocalization, direct interaction, physical, physical association	10747096, 12135674, 24287595, 7565724, (Europe PMC)	0.57	BioGRID, IntAct, MINT
PSTPIP1	Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex, anti bait coimmunoprecipitation, imaging technique	colocalization, physical, physical association	14707117, 19041431, 9488710, (Europe PMC)	0.46	BioGRID, IntAct, MINT
PTPN12	anti bait coimmunoprecipitation, phosphatase assay	dephosphorylation reaction, physical association	14707117, 19167335, (Europe PMC)	0.61	IntAct, MINT
SNX33	anti tag coimmunoprecipitation	physical association	19487689, (Europe PMC)	0.40	IntAct, MINT
SNX9	anti tag coimmunoprecipitation, two hybrid	physical association	17242350, 19487689, (Europe PMC)	0.57	IntAct, MINT
WBP4	Reconstituted Complex, pull down	physical, physical association	16055720, (Europe PMC)	0.40	BioGRID, IntAct, MINT

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Interacting partner	Detection method	Interaction type	PubMed IDs	Interaction Score	Source
ACTR2	pull down	association	12769847, (Europe PMC)	0.35	IntAct
ACTR3	pull down	association	12769847, (Europe PMC)	0.35	IntAct
APBB1	Reconstituted Complex	physical	16055720, (Europe PMC)	NA	BioGRID
APPBP2	Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical, physical association	25416956, (Europe PMC)	0.56	BioGRID, IntAct
ARPC1B	fluorescence microscopy, pull down	colocalization, direct interaction, physical association	10861055, 11943145, (Europe PMC)	0.64	IntAct, MINT
ARPC2	Reconstituted Complex	physical	11943145, (Europe PMC)	NA	BioGRID
ARPC3	Affinity Capture-MS, Reconstituted Complex, Two-hybrid, pull down	association, physical	12769847, 23148219, 9889097, (Europe PMC)	0.35	BioGRID, IntAct
ARPC4	Affinity Capture-MS, pull down	association, physical	12769847, (Europe PMC)	0.35	BioGRID, IntAct
BAIAP2L1	Reconstituted Complex	physical	22921828, (Europe PMC)	NA	BioGRID
BTK	Affinity Capture-Western, Reconstituted Complex, pull down	physical, physical association	10427990, 8892607, (Europe PMC)	0.40	BioGRID, IntAct
CBL	Affinity Capture-Western	physical	23684068, (Europe PMC)	NA	BioGRID
CBLB	Affinity Capture-Western	physical	23684068, (Europe PMC)	NA	BioGRID
CDC42	Affinity Capture-Western, Reconstituted Complex, Two-hybrid, circular dichroism, classical fluorescence spectroscopy, far western blotting, imaging technique, nuclear magnetic resonance, pull down, surface plasmon resonance, two hybrid pooling approach	direct interaction, physical, physical association	10360578, 10360579, 10684602, 10713100, 10724160, 15075243, 15361624, 20936779, 28539360, 8625410, 8643625, 9422512, 9660763, (Europe PMC)	0.44, 0.89	BioGRID, IntAct, MINT
CRKL	Affinity Capture-Western, Reconstituted Complex	physical	11313252, (Europe PMC)	NA	BioGRID
CSNK2A1	Biochemical Activity, protein kinase assay	phosphorylation reaction, physical	12769847, (Europe PMC)	0.44	BioGRID, IntAct
CTTN	anti bait coimmunoprecipitation, fluorescence microscopy	colocalization, physical association	15169891, (Europe PMC)	0.46	IntAct
DVL2	Two-hybrid, two hybrid	physical, physical association	21516116, (Europe PMC)	0.37	BioGRID, IntAct, MINT
EGFR	Affinity Capture-Western	physical	9307968, (Europe PMC)	NA	BioGRID
FCHSD2	Two-hybrid, two hybrid	physical, physical association	21988832, (Europe PMC)	0.37	BioGRID, IntAct
FGR	Affinity Capture-Western, Reconstituted Complex, pull down	direct interaction, physical	7565724, 8805332, 8824280, (Europe PMC)	0.44	BioGRID, IntAct, MINT
FNBP1	Affinity Capture-MS, Affinity Capture-Western	physical	16418535, 19041431, (Europe PMC)	NA	BioGRID
FYB1	Affinity Capture-Western	physical	10747096, (Europe PMC)	NA	BioGRID
FYN	Affinity Capture-Western, Biochemical Activity, Reconstituted Complex, anti bait coimmunoprecipitation, experimental interaction detection, pull down	direct interaction, phosphorylation reaction, physical, physical association	10532312, 12431372, 14707117, 7565724, 8805332, (Europe PMC)	0.70	BioGRID, IntAct, MINT
GAS7	Affinity Capture-Western, Reconstituted Complex, pull down	physical, physical association	16055720, 26506240, (Europe PMC)	0.40	BioGRID, IntAct, MINT
GRB2	Affinity Capture-Western, Reconstituted Complex, pull down	physical, physical association	8805332, 8892607, 9307968, (Europe PMC)	0.40	BioGRID, IntAct
HCK	Reconstituted Complex, anti bait coimmunoprecipitation, far western blotting, pull down	association, physical, physical association	12029088, 19234535, (Europe PMC)	0.72	BioGRID, IntAct, MINT
HCLS1	pull down	physical association	21398607, (Europe PMC)	0.40	IntAct
ITK	Reconstituted Complex, pull down	physical, physical association	8810341, 8892607, (Europe PMC)	0.40	BioGRID, IntAct
ITSN1	Affinity Capture-Western	physical	15824104, 18539136, (Europe PMC)	NA	BioGRID
ITSN2	Affinity Capture-Western, Reconstituted Complex, Two-hybrid	physical	11748279, 22558309, (Europe PMC)	NA	BioGRID
JMJD6	Affinity Capture-Western	physical	20574068, (Europe PMC)	NA	BioGRID
KDM4A	Affinity Capture-Western	physical	20574068, (Europe PMC)	NA	BioGRID
KDM6B	Affinity Capture-Western	physical	20574068, (Europe PMC)	NA	BioGRID
LCK	Affinity Capture-Western, Biochemical Activity, pull down	physical, physical association	16107303, 16293614, (Europe PMC)	0.40	BioGRID, IntAct, MINT
LCP2	Affinity Capture-Western	physical	10747096, (Europe PMC)	NA	BioGRID
LRPPRC	Affinity Capture-MS, anti tag coimmunoprecipitation	association, physical	26496610, (Europe PMC)	0.35	BioGRID, IntAct
NCK1	Affinity Capture-Western, Reconstituted Complex, coimmunoprecipitation, imaging technique, pull down	colocalization, direct interaction, physical, physical association	10747096, 12135674, 24287595, 7565724, (Europe PMC)	0.57	BioGRID, IntAct, MINT
NCK2	Affinity Capture-Western, Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical, physical association	24287595, 25416956, (Europe PMC)	0.56	BioGRID, IntAct
PACSIN1	Affinity Capture-MS	physical	19041431, (Europe PMC)	NA	BioGRID
PACSIN2	Affinity Capture-MS	physical	19041431, (Europe PMC)	NA	BioGRID
PACSIN3	Affinity Capture-MS	physical	19041431, (Europe PMC)	NA	BioGRID
PIK3R1	Affinity Capture-Western, Reconstituted Complex	physical	8805332, (Europe PMC)	NA	BioGRID
PLCG1	Reconstituted Complex, pull down	physical, physical association	8805332, 8824280, 8892607, (Europe PMC)	0.40	BioGRID, IntAct
PRPF40A	Reconstituted Complex	physical	16055720, (Europe PMC)	NA	BioGRID
PSTPIP1	Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex, anti bait coimmunoprecipitation, imaging technique	colocalization, physical, physical association	14707117, 19041431, 9488710, (Europe PMC)	0.46	BioGRID, IntAct, MINT
PTPN12	anti bait coimmunoprecipitation, phosphatase assay	dephosphorylation reaction, physical association	14707117, 19167335, (Europe PMC)	0.61	IntAct, MINT
PTPN22	phosphatase assay	dephosphorylation reaction	19167335, (Europe PMC)	0.44	IntAct
PTPRB	phosphatase assay	dephosphorylation reaction	19167335, (Europe PMC)	0.44	IntAct
PTPRC	phosphatase assay	dephosphorylation reaction	19167335, (Europe PMC)	0.44	IntAct
PTPRG	phosphatase assay	dephosphorylation reaction	19167335, (Europe PMC)	0.44	IntAct
PTPRJ	phosphatase assay	dephosphorylation reaction	19167335, (Europe PMC)	0.44	IntAct
PTPRO	phosphatase assay	dephosphorylation reaction	19167335, (Europe PMC)	0.44	IntAct
PTPRZ1	phosphatase assay	dephosphorylation reaction	19167335, (Europe PMC)	0.44	IntAct
RAC1	Reconstituted Complex	physical	8643625, (Europe PMC)	NA	BioGRID
RAC2	pull down	physical association	14521508, (Europe PMC)	0.40	IntAct
RAC3	pull down	physical association	14521508, (Europe PMC)	0.40	IntAct
RANBP2	Affinity Capture-MS, Affinity Capture-Western, Biochemical Activity	physical	26261240, (Europe PMC)	NA	BioGRID
RBBP5	Affinity Capture-Western	physical	20574068, (Europe PMC)	NA	BioGRID
RELA	Affinity Capture-Western	physical	26261240, (Europe PMC)	NA	BioGRID
RGS14	Two-hybrid, two hybrid	physical, physical association	21988832, (Europe PMC)	0.37	BioGRID, IntAct
RHOJ	Two-hybrid, pull down	physical, physical association	10967094, 14521508, (Europe PMC)	0.40	BioGRID, IntAct
RHOQ	pull down	physical association	14521508, (Europe PMC)	0.40	IntAct
ROBO4	two hybrid	physical association	18948384, (Europe PMC)	0.37	IntAct
SAE1	Affinity Capture-MS	physical	26261240, (Europe PMC)	NA	BioGRID
SNX33	anti tag coimmunoprecipitation	physical association	19487689, (Europe PMC)	0.40	IntAct, MINT
SNX9	anti tag coimmunoprecipitation, two hybrid	physical association	17242350, 19487689, (Europe PMC)	0.57	IntAct, MINT
SORBS2	Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical, physical association	25416956, (Europe PMC)	0.56	BioGRID, IntAct
SRC	Affinity Capture-Western, Reconstituted Complex	physical	8805332, 8824280, (Europe PMC)	NA	BioGRID
SRGAP2	Affinity Capture-MS	physical	19041431, (Europe PMC)	NA	BioGRID
TCERG1	Reconstituted Complex	physical	16055720, (Europe PMC)	NA	BioGRID
TEC	Reconstituted Complex, pull down	physical, physical association	8892607, (Europe PMC)	0.40	BioGRID, IntAct
TNK2	Affinity Capture-Western, Biochemical Activity	physical	16257963, (Europe PMC)	NA	BioGRID
TRIP10	Affinity Capture-Western, Reconstituted Complex, Two-hybrid	physical	10713100, 21516116, (Europe PMC)	NA	BioGRID
UBA2	Affinity Capture-MS, Affinity Capture-Western	physical	26261240, (Europe PMC)	NA	BioGRID
UBE2I	Affinity Capture-MS, Affinity Capture-Western	physical	26261240, (Europe PMC)	NA	BioGRID
VASP	Reconstituted Complex	physical	11598004, (Europe PMC)	NA	BioGRID
WAS	classical fluorescence spectroscopy, pull down	direct interaction, physical association	10724160, 12769847, 18650809, (Europe PMC)	0.75	IntAct
WBP4	Reconstituted Complex, pull down	physical, physical association	16055720, (Europe PMC)	0.40	BioGRID, IntAct, MINT
WIPF1	Affinity Capture-Western, Reconstituted Complex, Two-hybrid, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, pull down, two hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical, physical association	10202051, 16488394, 17213309, 19805221, 21988832, 25416956, 9405671, 9694849, (Europe PMC)	0.40, 0.49, 0.95	BioGRID, IntAct
WIPF2	Affinity Capture-Western, Two-hybrid, two hybrid	physical, physical association	12213210, 21988832, (Europe PMC)	0.37	BioGRID, IntAct

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WAS