DEPOD - human DEPhOsphorylation Database

Basic Information
Phosphatases
Pathway
Interacting Proteins
Phosphorylating Kinases

Gene Name	CRK (QuickGO)	Interactive visualization of CRK structures (A quick tutorial to explore the interctive visulaization) Representative structure: 5UL6
Synonyms	CRK
Protein Name	CRK
Alternative Name(s)	Adapter molecule crk;Proto-oncogene c-Crk;p38;
Protein Family	Belongs to the CRK family
EntrezGene ID	1398 (Comparitive Toxicogenomics)
UniProt AC (Human)	P46108 (protein sequence)
Enzyme Class	N/A
Molecular Weight	33831 Dalton
Protein Length	304 amino acids (AA)
Genome Browsers	NCBI \| ENSG00000167193 (Ensembl) \| UCSC
Crosslinking annotations	Query our ID-mapping table
Orthologues	Quest For Orthologues (QFO) \| GeneTree \| eggNOG - KOG4792 \| eggNOG - ENOG4110574
Phosphorylation Network	Visualize

Domain organization, Expression, Diseases

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Localization, Function, Catalytic activity and Sequence

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Motif information from Eukaryotic Linear Motif atlas (ELM)

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Gene Ontology (P: Process; F: Function and C: Component terms)

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GO:0000186	P:activation of MAPKK activity
GO:0001784	F:phosphotyrosine residue binding
GO:0001878	P:response to yeast
GO:0005070	F:SH3/SH2 adaptor activity
GO:0005159	F:insulin-like growth factor receptor binding
GO:0005634	C:nucleus
GO:0005737	C:cytoplasm
GO:0005829	C:cytosol
GO:0005886	C:plasma membrane
GO:0006357	P:regulation of transcription by RNA polymerase II
GO:0008092	F:cytoskeletal protein binding
GO:0008360	P:regulation of cell shape
GO:0009966	P:regulation of signal transduction
GO:0014911	P:positive regulation of smooth muscle cell migration
GO:0015629	C:actin cytoskeleton
GO:0017124	F:SH3 domain binding
GO:0019221	P:cytokine-mediated signaling pathway
GO:0030307	P:positive regulation of cell growth
GO:0032956	P:regulation of actin cytoskeleton organization
GO:0032991	C:protein-containing complex
GO:0035020	P:regulation of Rac protein signal transduction
GO:0035728	P:response to hepatocyte growth factor
GO:0038096	P:Fc-gamma receptor signaling pathway involved in phagocytosis
GO:0042169	F:SH2 domain binding
GO:0042542	P:response to hydrogen peroxide
GO:0043087	P:regulation of GTPase activity
GO:0043393	P:regulation of protein binding
GO:0043621	F:protein self-association
GO:0045121	C:membrane raft
GO:0045309	F:protein phosphorylated amino acid binding
GO:0045953	P:negative regulation of natural killer cell mediated cytotoxicity
GO:0046875	F:ephrin receptor binding
GO:0048010	P:vascular endothelial growth factor receptor signaling pathway
GO:0048013	P:ephrin receptor signaling pathway
GO:0061045	P:negative regulation of wound healing
GO:0061847	P:response to cholecystokinin
GO:0070062	C:extracellular exosome
GO:0071538	P:SH2 domain-mediated complex assembly
GO:0071560	P:cellular response to transforming growth factor beta stimulus
GO:0071732	P:cellular response to nitric oxide
GO:0097110	F:scaffold protein binding
GO:1900026	P:positive regulation of substrate adhesion-dependent cell spreading
GO:1990090	P:cellular response to nerve growth factor stimulus
GO:1990314	P:cellular response to insulin-like growth factor stimulus
GO:1990782	F:protein tyrosine kinase binding
GO:1990859	P:cellular response to endothelin
GO:2000146	P:negative regulation of cell motility

KEGG Pathway
Reactome Pathway

Pathway ID	Pathway Name	Pathway Description (KEGG)
hsa04010	MAPK signaling pathway	The mitogen-activated protein kinase (MAPK) cascade is a highly conserved module that is involved in various cellular functions, including cell proliferation, differentiation and migration. Mammals express at least four distinctly regulated groups of MAPKs, extracellular signal-related kinases (ERK)-1/2, Jun amino-terminal kinases (JNK1/2/3), p38 proteins (p38alpha/beta/gamma/delta) and ERK5, that are activated by specific MAPKKs: MEK1/2 for ERK1/2, MKK3/6 for the p38, MKK4/7 (JNKK1/2) for the JNKs, and MEK5 for ERK5. Each MAPKK, however, can be activated by more than one MAPKKK, increasing the complexity and diversity of MAPK signalling. Presumably each MAPKKK confers responsiveness to distinct stimuli. For example, activation of ERK1/2 by growth factors depends on the MAPKKK c-Raf, but other MAPKKKs may activate ERK1/2 in response to pro-inflammatory stimuli.
hsa04012	ErbB signaling pathway	The ErbB family of receptor tyrosine kinases (RTKs) couples binding of extracellular growth factor ligands to intracellular signaling pathways regulating diverse biologic responses, including proliferation, differentiation, cell motility, and survival. Ligand binding to the four closely related members of this RTK family -epidermal growth factor receptor (EGFR, also known as ErbB-1 or HER1), ErbB-2 (HER2), ErbB-3 (HER3), and ErbB-4 (HER4)-induces the formation of receptor homo- and heterodimers and the activation of the intrinsic kinase domain, resulting in phosphorylation on specific tyrosine residues (pY) within the cytoplasmic tail. Signaling effectors containing binding pockets for pY-containing peptides are recruited to activated receptors and induce the various signaling pathways. The Shc- and/or Grb2-activated mitogen-activated protein kinase (MAPK) pathway is a common target downstream of all ErbB receptors. Similarly, the phosphatidylinositol-3-kinase (PI-3K) pathway is directly or indirectly activated by most ErbBs. Several cytoplasmic docking proteins appear to be recruited by specific ErbB receptors and less exploited by others. These include the adaptors Crk, Nck, the phospholipase C gamma (PLCgamma), the intracellular tyrosine kinase Src, or the Cbl E3 ubiquitin protein ligase.
hsa04015	Rap1 signaling pathway	Rap1 is a small GTPase that controls diverse processes, such as cell adhesion, cell-cell junction formation and cell polarity. Like all G proteins, Rap1 cycles between an inactive GDP-bound and an active GTP-bound conformation. A variety of extracellular signals control this cycle through the regulation of several unique guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). Rap1 plays a dominant role in the control of cell-cell and cell-matrix interactions by regulating the function of integrins and other adhesion molecules in various cell types. Rap1 also regulates MAP kinase (MAPK) activity in a manner highly dependent on the context of cell types.
hsa04062	Chemokine signaling pathway	Inflammatory immune response requires the recruitment of leukocytes to the site of inflammation upon foreign insult. Chemokines are small chemoattractant peptides that provide directional cues for the cell trafficking and thus are vital for protective host response. In addition, chemokines regulate plethora of biological processes of hematopoietic cells to lead cellular activation, differentiation and survival.The chemokine signal is transduced by chemokine receptors (G-protein coupled receptors) expressed on the immune cells. After receptor activation, the alpha- and beta-gamma-subunits of G protein dissociate to activate diverse downstream pathways resulting in cellular polarization and actin reorganization. Various members of small GTPases are involved in this process. Induction of nitric oxide and production of reactive oxygen species are as well regulated by chemokine signal via calcium mobilization and diacylglycerol production.
hsa04510	Focal adhesion	Cell-matrix adhesions play essential roles in important biological processes including cell motility, cell proliferation, cell differentiation, regulation of gene expression and cell survival. At the cell-extracellular matrix contact points, specialized structures are formed and termed focal adhesions, where bundles of actin filaments are anchored to transmembrane receptors of the integrin family through a multi-molecular complex of junctional plaque proteins. Some of the constituents of focal adhesions participate in the structural link between membrane receptors and the actin cytoskeleton, while others are signalling molecules, including different protein kinases and phosphatases, their substrates, and various adapter proteins. Integrin signaling is dependent upon the non-receptor tyrosine kinase activities of the FAK and src proteins as well as the adaptor protein functions of FAK, src and Shc to initiate downstream signaling events. These signalling events culminate in reorganization of the actin cytoskeleton; a prerequisite for changes in cell shape and motility, and gene expression. Similar morphological alterations and modulation of gene expression are initiated by the binding of growth factors to their respective receptors, emphasizing the considerable crosstalk between adhesion- and growth factor-mediated signalling.
hsa04666	Fc gamma R-mediated phagocytosis	Phagocytosis plays an essential role in host-defense mechanisms through the uptake and destruction of infectious pathogens. Specialized cell types including macrophages, neutrophils, and monocytes take part in this process in higher organisms. After opsonization with antibodies (IgG), foreign extracellular materials are recognized by Fc gamma receptors. Cross-linking of Fc gamma receptors initiates a variety of signals mediated by tyrosine phosphorylation of multiple proteins, which lead through the actin cytoskeleton rearrangements and membrane remodeling to the formation of phagosomes. Nascent phagosomes undergo a process of maturation that involves fusion with lysosomes. The acquisition of lysosomal proteases and release of reactive oxygen species are crucial for digestion of engulfed materials in phagosomes.
hsa04722	Neurotrophin signaling pathway	Neurotrophins are a family of trophic factors involved in differentiation and survival of neural cells. The neurotrophin family consists of nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3), and neurotrophin 4 (NT-4). Neurotrophins exert their functions through engagement of Trk tyrosine kinase receptors or p75 neurotrophin receptor (p75NTR). Neurotrophin/Trk signaling is regulated by connecting a variety of intracellular signaling cascades, which include MAPK pathway, PI-3 kinase pathway, and PLC pathway, transmitting positive signals like enhanced survival and growth. On the other hand, p75NTR transmits both positive and nagative signals. These signals play an important role for neural development and additional higher-order activities such as learning and memory.
hsa04810	Regulation of actin cytoskeleton
hsa04910	Insulin signaling pathway	Insulin binding to its receptor results in the tyrosine phosphorylation of insulin receptor substrates (IRS) by the insulin receptor tyrosine kinase (INSR). This allows association of IRSs with the regulatory subunit of phosphoinositide 3-kinase (PI3K). PI3K activates 3-phosphoinositide-dependent protein kinase 1 (PDK1), which activates Akt, a serine kinase. Akt in turn deactivates glycogen synthase kinase 3 (GSK-3), leading to activation of glycogen synthase (GYS) and thus glycogen synthesis. Activation of Akt also results in the translocation of GLUT4 vesicles from their intracellular pool to the plasma membrane, where they allow uptake of glucose into the cell. Akt also leads to mTOR-mediated activation of protein synthesis by eIF4 and p70S6K. The translocation of GLUT4 protein is also elicited through the CAP/Cbl/TC10 pathway, once Cbl is phosphorylated by INSR.Other signal transduction proteins interact with IRS including GRB2. GRB2 is part of the cascade including SOS, RAS, RAF and MEK that leads to activation of mitogen-activated protein kinase (MAPK) and mitogenic responses in the form of gene transcription. SHC is another substrate of INSR. When tyrosine phosphorylated, SHC associates with GRB2 and can thus activate the RAS/MAPK pathway independently of IRS-1.
hsa05100	Bacterial invasion of epithelial cells	Many pathogenic bacteria can invade phagocytic and non-phagocytic cells and colonize them intracellularly, then become disseminated to other cells. Invasive bacteria induce their own uptake by non-phagocytic host cells (e.g. epithelial cells) using two mechanisms referred to as zipper model and trigger model. Listeria, Staphylococcus, Streptococcus, and Yersinia are examples of bacteria that enter using the zipper model. These bacteria express proteins on their surfaces that interact with cellular receptors, initiating signalling cascades that result in close apposition of the cellular membrane around the entering bacteria. Shigella and Salmonella are the examples of bacteria entering cells using the trigger model. These bacteria use type III secretion systems to inject protein effectors that interact with the actin cytoskeleton.
hsa05131	Shigellosis	Shigellosis, or bacillary dysentery, is an intestinal infection caused by Shigella, a genus of enterobacteria. Shigella are potential food-borne pathogens that are capable of colonizing the intestinal epithelium by exploiting epithelial-cell functions and circumventing the host innate immune response. During basolateral entry into the host-cell cytoplasm, Shigella deliver a subset of effectors into the host cells through the type III secretion system. The effectors induce membrane ruffling through the stimulation of the Rac1-WAVE-Arp2/3 pathway, enabling bacterial entry into the epithelial cells. During multiplication within the cells, Shigella secrete another subset of effectors. VirG induces actin polymerization at one pole of the bacteria, allowing the bacteria to spread intracellularly and to infect adjacent cells. OspF, OspG and IpaH(9.8) downregulate the production of proinflammatory cytokines such as IL-8, helping bacteria circumvent the innate immune response.
hsa05163	Human cytomegalovirus infection	Human cytomegalovirus (HCMV) is an enveloped, double-stranded DNA virus that is a member of beta-herpesvirus family. HCMV is best known for causing significant morbidity and mortality in immunocompromised populations. As with other herpesviruses, HCMV gB and gH/gL envelope glycoproteins are essential for virus entry. HCMV gB could activate the PDGFRA, and induce activation of the oncogenic PI3-K/AKT pathway. Though it is unlikely that HCMV by itself can act as an oncogenic factor, HCMV may have an oncomodulatory role, to catalyze an oncogenic process that has already been initiated. US28, one of the four HCMV-encoded vGPCRs (US27, US28, UL33 and UL78), also has a specific role in the oncomodulatory properties. In addition, HCMV has developed numerous mechanisms for manipulating the host immune system. The virally encoded US2, US3, US6 and US11 gene products all interfere with major histocompatibility complex (MHC) class I antigen presentation. HCMV encodes several immediate early (IE) antiapoptotic proteins (IE1, IE2, vMIA and vICA). These proteins might avoid immune clearance of infected tumor cells by cytotoxic lymphocytes and NK cells.
hsa05170	Human immunodeficiency virus 1 infection	Human immunodeficiency virus type 1 (HIV-1) , the causative agent of AIDS (acquired immunodeficiency syndrome), is a lentivirus belonging to the Retroviridae family. The primary cell surface receptor for HIV-1, the CD4 protein, and the co-receptor for HIV-1, either CCR5 or CXCR4, are found on macrophages and T lymphocytes. At the earliest step, sequential binding of virus envelope (Env) glycoprotein gp120 to CD4 and the co-receptor CCR5 or CXCR4 facilitates HIV-1 entry and has the potential to trigger critical signaling that may favor viral replication. At advanced stages of the disease, HIV-1 infection results in dramatic induction of T-cell (CD4+ T and CD8+ T cell) apoptosis both in infected and uninfected bystander T cells, a hallmark of HIV-1 pathogenesis. On the contrary, macrophages are resistant to the cytopathic effect of HIV-1 and produce virus for longer periods of time.
hsa05200	Pathways in cancer
hsa05206	MicroRNAs in cancer	MicroRNA (miRNA) is a cluster of small non-encoding RNA molecules of 21 - 23 nucleotides in length, which controls gene expression post-transcriptionally either via the degradation of target mRNAs or the inhibition of protein translation. Using high-throughput profiling, dysregulation of miRNAs has been widely observed in different stages of cancer. The upregulation (overexpression) of specific miRNAs could lead to the repression of tumor suppressor gene expression, and conversely the downregulation of specific miRNAs could result in an increase of oncogene expression; both these situations induce subsequent malignant effects on cell proliferation, differentiation, and apoptosis that lead to tumor growth and progress. The miRNA signatures of cancer observed in various studies differ significantly. These inconsistencies occur due to the differences in the study populations and methodologies used. This pathway map shows the summarized results from various studies in 9 cancers, each of which is presented in a review article.
hsa05211	Renal cell carcinoma	Renal cell cancer (RCC) accounts for ~3% of human malignancies and its incidence appears to be rising. Although most cases of RCC seem to occur sporadically, an inherited predisposition to renal cancer accounts for 1-4% of cases. RCC is not a single disease, it has several morphological subtypes. Conventional RCC (clear cell RCC) accounts for ~80% of cases, followed by papillary RCC (10-15%), chromophobe RCC (5%), and collecting duct RCC (<1%). Genes potentially involved in sporadic neoplasms of each particular type are VHL, MET, BHD, and FH respectively. In the absence of VHL, hypoxia-inducible factor alpha (HIF-alpha) accumulates, leading to production of several growth factors, including vascular endothelial growth factor and platelet-derived growth factor. Activated MET mediates a number of biological effects including motility, invasion of extracellular matrix, cellular transformation, prevention of apoptosis and metastasis formation. Loss of functional FH leads to accumulation of fumarate in the cell, triggering inhibition of HPH and preventing targeted pVHL-mediated degradation of HIF-alpha. BHD mutations cause the Birt-Hogg-Dube syndrome and its associated chromophobe, hybrid oncocytic, and conventional (clear cell) RCC.
hsa05220	Chronic myeloid leukemia	Chronic myeloid leukemia (CML) is a clonal myeloproliferative disorder of a pluripotent stem cell. The natural history of CML has a triphasic clinical course comprising of an initial chronic phase (CP), which is characterized by expansion of functionally normal myeloid cells, followed by an accelerated phase (AP) and finally a more aggressive blast phase (BP), with loss of terminal differentiation capacity. On the cellular level, CML is associated with a specific chromosome abnormality, the t(9; 22) reciprocal translocation that forms the Philadelphia (Ph) chromosome. The Ph chromosome is the result of a molecular rearrangement between the c-ABL proto-oncogene on chromosome 9 and the BCR (breakpoint cluster region) gene on chromosome 22. The BCR/ABL fusion gene encodes p210 BCR/ABL, an oncoprotein, which, unlike the normal p145 c-Abl, has constitutive tyrosine kinase activity and is predominantly localized in the cytoplasm. While fusion of c-ABL and BCR is believed to be the primary cause of the chronic phase of CML, progression to blast crisis requires other molecular changes. Common secondary abnormalities include mutations in TP53, RB, and p16/INK4A, or overexpression of genes such as EVI1. Additional chromosome translocations are also observed,such as t(3;21)(q26;q22), which generates AML1-EVI1.

Pathway ID	Pathway Name	Pathway Description (KEGG)
R-HSA-170984	ARMS-mediated activation.	ARMS (Ankyrin-Rich Membrane Spanning/Kidins 220) is a 220kD tetraspanning adaptor protein which becomes rapidly tyrosine phosphorylated by active Trk receptors. ARMS is another adaptor protein which is involved in the activation of Rap1 and the subsequent prolonged activation of the MAPK cascade
R-HSA-186763	Downstream signal transduction.	The role of autophosphorylation sites on PDGF receptors are to provide docking sites for downstream signal transduction molecules which contain SH2 domains. The SH2 domain is a conserved motif of around 100 amino acids that can bind a phosphorylated tyrosine residue. These downstream molecules are activated upon binding to, or phosphorylated by, the receptor kinases intrinsic to PDGF receptors.Some of the dowstream molecules are themselves enzymes, such as phosphatidylinositol 3'-kinase (PI3K), phospholipase C (PLC-gamma), the Src family of tyrosine kinases, the tyrosine phosphatase SHP2, and a GTPase activating protein (GAP) for Ras. Others such as Grb2 are adaptor molecules which link the receptor with downstream catalytic molecules
R-HSA-2029482	Regulation of actin dynamics for phagocytic cup formation.	The actin cytoskeleton is fundamental for phagocytosis and members of the Rho family GTPases RAC and CDC42 are involved in actin cytoskeletal regulation leading to pseudopod extension. Active RAC and CDC42 exert their action through the members of WASP family proteins (WASP/N-WASP/WAVE) and ARP2/3 complex. Actin filaments move from the bottom toward the top of the phagocytic cup during pseudopod extension
R-HSA-372708	p130Cas linkage to MAPK signaling for integrins.	Integrin signaling is linked to the MAP kinase pathway by recruiting p130cas and Crk to the FAK/Src activation complex
R-HSA-4420097	VEGFA-VEGFR2 Pathway.	Angiogenesis is the formation of new blood vessels from preexisting vasculature. One of the most important proangiogenic factors is vascular endothelial growth factor (VEGF). VEGF exerts its biologic effect through interaction with transmembrane tyrosine kinase receptors VEGFR, selectively expressed on vascular endothelial cells. VEGFA signaling through VEGFR2 is the major pathway that activates angiogenesis by inducing the proliferation, survival, sprouting and migration of endothelial cells (ECs), and also by increasing endothelial permeability (Lohela et al. 2009, Shibuya & Claesson-Welsh 2006, Claesson-Welsh & Welsh, 2013). The critical role of VEGFR2 in vascular development is highlighted by the fact that VEGFR2-/- mice die at E8.5-9.5 due to defective development of blood islands, endothelial cells and haematopoietic cells (Shalaby et al. 1995)
R-HSA-8849471	PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.	PTK6 promotes cell motility and migration by regulating the activity of RHO GTPases RAC1 (Chen et al. 2004) and RHOA (Shen et al. 2008). PTK6 inhibits RAS GTPase activating protein RASA1 (Shen et al. 2008) and may be involved in MAPK7 (ERK5) activation (Ostrander et al. 2007, Zheng et al
R-HSA-8875555	MET activates RAP1 and RAC1.	The adapter protein GAB1 is involved in recruitment, through CRK and related CRKL proteins, of guanyl nucleotide exchange factors (GEFs) to the activated MET receptor. MET-associated GEFs, such as RAPGEF1 (C3G) and DOCK7, activate RAP1 and RAC1, respectively, leading to morphological changes that contribute to cell motility (Schaeper et al. 2000, Sakkab et al. 2000, Lamorte et al. 2002, Watanabe et al. 2006, Murray et al. 2014)
R-HSA-8875656	MET receptor recycling.	Activated MET receptor is subject to recycling from the plasma membrane through the endosomal compartment and back to the plasma membrane (Peschard et al. 2001, Hammond et al. 2001, Petrelli et al. 2002). In the recycling process, activated MET receptor is endocytosed, and the GGA3 protein directs it, via a largely unknown mechanism, through the RAB4 positive endosomal compartments back to the plasma membrane (Parachoniak et al. 2011). Endosomal signaling by MET during the recycling process appears to play an important role in sustained activation of ERK1/ERK2 (MAPK3/MAPK1) and STAT3 downstream of MET (Kermorgant and Parker 2008)
R-HSA-912631	Regulation of signaling by CBL.	Cbl is an E3 ubiquitin-protein ligase that negatively regulates signaling pathways by targeting proteins for ubiquitination and proteasomal degradation (Rao et al. 2002). Cbl negatively regulates PI3K via this mechanism (Dufour et al. 2008). The binding of Cbl to the p85 subunit of PI3K is mediated at least in part by tyrosine phosphorylation at Y731 (Dufour et al. 2008). Fyn and the related kinases Hck and Lyn are known to be associated with Cbl (Anderson et al. 1997, Hunter et al. 1999). Fyn is proven capable of Cbl Y731 phosphorylation (Hunter et al. 1999).The association of Fyn and Cbl has been described as constitutive (Hunter et al. 1999). CBL further associates with the p85 subunit of PI3K (Hartley et al. 1995, Anderson et al. 1997, Hunter et al. 1997), this also described as constitutive and mediated by the SH3 domain of p85. Binding of the SH2 domain of p85 to a specific phosphorylation site in Cbl is postulated to explain the the increase in Cbl/p85 association seen in activated cells (Panchamoorthy et al 1996) which negatively regulates PI3K activity (Fang et al. 2001). The negative effect of increased Cbl-PI3K interaction is mediated by Y731 of Cbl. Cbl binding increases PI3K ubiquitination and proteasome degradation (Dufour et al. 2008).Cbl is constitutively associated with Grb in resting hematopoietic cells (Anderson et al. 1997, Odai et al. 1995, Park et al. 1998, Panchamoorthy et al. 1996). Both the SH2 and SH3 domains of Grb2 are involved. Cbl has 2 distinct C-terminal domains, proximal and distal. The proximal domain binds Grb2 in resting and stimulated cells, and in stimulated cells also binds Shc. The distal domain binds the adaptor protein CRKL. Tyrosine phosphorylation of Cbl in response to IL-3 releases the SH3 domain of Grb2 which then is free to bind other molecules (Park et al. 1998). Cbl is tyrosine phosphorylated in response to many cytokines including IL-3, IL-2 (Gesbert et al. 1998) and IL-4 (Ueno et al. 1998)

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Interacting partner	Detection method	Interaction type	PubMed IDs	Interaction Score	Source
ABL2	Affinity Capture-MS, Biochemical Activity, Reconstituted Complex, Two-hybrid, peptide array, protein kinase assay, tandem affinity purification, two hybrid array, validated two hybrid	association, phosphorylation reaction, physical, physical association	15886098, 17474147, 19380743, 25814554, 8194526, (Europe PMC)	0.49, 0.70	BioGRID, IntAct
ACTB	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
AGO1	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
AGO2	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
ANKZF1	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
ANLN	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
AP2A1	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
AP2B1	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
AP2M1	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
ARHGAP17	Reconstituted Complex	physical	11431473, (Europe PMC)	NA	BioGRID
ARHGAP32	Affinity Capture-Western, Reconstituted Complex	physical	12454018, 12819203, (Europe PMC)	NA	BioGRID
ARPC1B	Affinity Capture-MS, anti tag coimmunoprecipitation	association, physical	26496610, (Europe PMC)	0.35	BioGRID, IntAct
ASAP1	Affinity Capture-MS, Reconstituted Complex, peptide array	physical, physical association	17474147, 19380743, 9819391, (Europe PMC)	0.40	BioGRID, IntAct
ASAP3	Affinity Capture-MS, bimolecular fluorescence complementation, tandem affinity purification, two hybrid array, two hybrid prey pooling approach, validated two hybrid	association, physical, physical association	19380743, 26871637, (Europe PMC)	0.70	BioGRID, IntAct
ASB9	Affinity Capture-Luminescence, Two-hybrid, luminescence based mammalian interactome mapping, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.51	BioGRID, IntAct
ASCL4	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
ATF3	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
ATIC	Co-fractionation	physical	22939629, (Europe PMC)	NA	BioGRID
ATXN1	Affinity Capture-Western, Two-hybrid, pull down, two hybrid array, two hybrid prey pooling approach	physical, physical association	16713569, 23275563, (Europe PMC)	0.63	BioGRID, IntAct
AVIL	Reconstituted Complex	physical	11287316, (Europe PMC)	NA	BioGRID
BAG3	Affinity Capture-Luminescence, Affinity Capture-MS	physical	23824909, (Europe PMC)	NA	BioGRID
BATF3	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
BCAR1	Affinity Capture-MS, Affinity Capture-Western, Protein-peptide, Reconstituted Complex, anti bait coimmunoprecipitation, pull down	association, physical, physical association	10085298, 10329689, 10464310, 10747099, 10753828, 11278916, 11369773, 11839772, 12615911, 12799422, 14657239, 15492270, 15673687, 16267043, 17038317, 18835194, 19380743, 22974441, 9581808, 9920935, (Europe PMC)	0.76	BioGRID, IntAct, MINT
BCR	Affinity Capture-MS, Reconstituted Complex	physical	19380743, 8943292, (Europe PMC)	NA	BioGRID
BEX5	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
BUB1	Two-hybrid, two hybrid pooling approach	physical, physical association	16189514, (Europe PMC)	0.37	BioGRID, IntAct
C4orf17	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
C6orf141	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
CASS4	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
CAST	Co-fractionation, peptide array	physical, physical association	17474147, 26344197, (Europe PMC)	0.40	BioGRID, IntAct
CBL	Affinity Capture-MS, Affinity Capture-Western, Protein-peptide, Reconstituted Complex, Two-hybrid, anti bait coimmunoprecipitation, coimmunoprecipitation, pull down, tandem affinity purification, two hybrid array, validated two hybrid	association, physical, physical association	10962563, 11792427, 12393469, 15581361, 16289966, 18835194, 19380743, 22974441, 25814554, 8524328, 8621483, 8626404, 8626543, 8662998, 8683103, 8943292, 9129019, 9178909, 9416834, 9461587, 9617486, 9988765, (Europe PMC)	0.49, 0.88	BioGRID, IntAct, MINT
CBLB	Affinity Capture-MS, Two-hybrid, tandem affinity purification, two hybrid array, validated two hybrid	association, physical, physical association	19380743, 25814554, (Europe PMC)	0.49, 0.62	BioGRID, IntAct
CBLC	Reconstituted Complex, Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	10362357, 25814554, (Europe PMC)	0.49	BioGRID, IntAct
CCP110	Proximity Label-MS, proximity-dependent biotin identification	association, physical	26638075, (Europe PMC)	0.35	BioGRID, IntAct
CDH1	Proximity Label-MS	physical	25468996, (Europe PMC)	NA	BioGRID
CEP128	Proximity Label-MS, proximity-dependent biotin identification	association, physical	26638075, (Europe PMC)	0.35	BioGRID, IntAct
CEP19	Proximity Label-MS, proximity-dependent biotin identification	association, physical	26638075, (Europe PMC)	0.35	BioGRID, IntAct
CEP89	Proximity Label-MS, proximity-dependent biotin identification	association, physical	26638075, (Europe PMC)	0.35	BioGRID, IntAct
CHTF18	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
CLNK	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
CNDP2	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
CORO1C	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
CORO6	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
COX6C	Affinity Capture-MS, anti tag coimmunoprecipitation	association, physical	26496610, (Europe PMC)	0.35	BioGRID, IntAct
CSE1L	Affinity Capture-Western	physical	8943292, (Europe PMC)	NA	BioGRID
DDX3X	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
DDX5	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
DDX6	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
DHX9	Co-fractionation	physical	22939629, (Europe PMC)	NA	BioGRID
DNAJA3	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
DOCK1	Affinity Capture-Western, Co-fractionation, Reconstituted Complex, peptide array, pull down	association, physical, physical association	10559471, 11369773, 12615911, 15673687, 17474147, 17515907, 26344197, 8657152, 8662907, (Europe PMC)	0.35, 0.40	BioGRID, IntAct
DOK1	Protein-peptide	physical	22974441, (Europe PMC)	NA	BioGRID
DOK2	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
DOK3	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
DOK7	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
DPPA4	Two-hybrid, two hybrid array, two hybrid pooling approach, two hybrid prey pooling approach, validated two hybrid	physical, physical association	16189514, 26871637, (Europe PMC)	0.67	BioGRID, IntAct
DSC1	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
EDC4	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
EEF1A1	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
EFS	Far Western, peptide array	physical, physical association	17474147, 8647432, (Europe PMC)	0.40	BioGRID, IntAct
EGFR	Affinity Capture-Luminescence, Affinity Capture-MS, Affinity Capture-Western, Biochemical Activity, PCA, Protein-peptide, Two-hybrid, luminescence based mammalian interactome mapping, protein array, ubiquitin reconstruction	direct interaction, physical, physical association	10595738, 16273093, 16729043, 24658140, 25402006, 9617486, 9642287, (Europe PMC)	0.51, 0.59	BioGRID, IntAct, MINT
ELK1	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
EPHA3	Affinity Capture-Western	physical	11870224, (Europe PMC)	NA	BioGRID
EPHB2	Reconstituted Complex	physical	10644995, (Europe PMC)	NA	BioGRID
EPHB3	Reconstituted Complex	physical	9674711, (Europe PMC)	NA	BioGRID
EPS15	Affinity Capture-MS, Far Western, Reconstituted Complex, tandem affinity purification	association, physical	19380743, 7797522, (Europe PMC)	0.35	BioGRID, IntAct
EPYC	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
ERBB2	Protein-peptide, protein array	direct interaction, physical	16273093, (Europe PMC)	0.44	BioGRID, IntAct, MINT
ERBB3	Protein-peptide, protein array	direct interaction, physical	16273093, (Europe PMC)	0.44	BioGRID, IntAct, MINT
ERBB4	Affinity Capture-MS	physical	16729043, (Europe PMC)	NA	BioGRID
ESD	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
EYA3	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
FASLG	Protein-peptide, phage display	direct interaction, physical	19807924, (Europe PMC)	0.44	BioGRID, IntAct
FER	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
FGFR1	Affinity Capture-Western, Co-localization, Reconstituted Complex, affinity chromatography technology, proximity ligation assay	association, physical, physical association	10464310, 25241761, (Europe PMC)	0.56	BioGRID, IntAct, MINT
FLNA	Co-localization, peptide array, proximity ligation assay	physical, physical association	17474147, 25241761, (Europe PMC)	0.59	BioGRID, IntAct
FRS2	Reconstituted Complex	physical	10092678, (Europe PMC)	NA	BioGRID
FSTL1	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
FUBP1	Co-fractionation	physical	22939629, (Europe PMC)	NA	BioGRID
GAB1	Affinity Capture-MS, fluorescence polarization spectroscopy	direct interaction, physical	19380743, 24728074, (Europe PMC)	0.44	BioGRID, IntAct
GAB2	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
GABPB2	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
GAREM1	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
GFPT1	Co-fractionation	physical	26344197, (Europe PMC)	NA	BioGRID
GGA3	Affinity Capture-Western	physical	21664574, (Europe PMC)	NA	BioGRID
GRB2	Affinity Capture-MS, Affinity Capture-Western, Far Western, Reconstituted Complex, tandem affinity purification	association, physical	10962563, 10970810, 19380743, 8662907, 8810325, (Europe PMC)	0.35	BioGRID, IntAct
HABP4	Two-hybrid	physical	25814554, (Europe PMC)	NA	BioGRID
HIST1H1D	Affinity Capture-MS	physical	26496610, (Europe PMC)	NA	BioGRID
HSH2D	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
HSP90AB1	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
HSPA1B	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
HSPA1L	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
HSPA5	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
HSPA6	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
HSPA8	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
HSPA9	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
HSPB1	Co-fractionation	physical	22939629, (Europe PMC)	NA	BioGRID
HSPBP1	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
HUWE1	Affinity Capture-MS	physical	25147182, (Europe PMC)	NA	BioGRID
IFT140	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
INF2	Affinity Capture-MS, anti tag coimmunoprecipitation	association, physical	26496610, (Europe PMC)	0.35	BioGRID, IntAct
INO80E	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
INPPL1	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
IRS4	Affinity Capture-Western, Reconstituted Complex, coimmunoprecipitation, pull down	association, physical	11316748, 9614078, (Europe PMC)	0.46	BioGRID, IntAct, MINT
ISL1	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
KAT6A	Affinity Capture-MS, anti tag coimmunoprecipitation	association, physical	26496610, (Europe PMC)	0.35	BioGRID, IntAct
KCTD13	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
KCTD17	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
KHDRBS1	Reconstituted Complex	physical	1545818, (Europe PMC)	NA	BioGRID
KIT	Two-hybrid, fluorescence polarization spectroscopy, pull down	association, direct interaction, physical	12878163, 24728074, 9233773, (Europe PMC)	0.59	BioGRID, IntAct, MINT
KLF15	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
KLHL20	Two-hybrid	physical	25814554, (Europe PMC)	NA	BioGRID
KRT1	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT10	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT12	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT14	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT15	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT16	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT17	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT19	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT2	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT24	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT3	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT4	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT5	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT6A	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT6B	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT73	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT76	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT77	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT78	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT79	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT8	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
KRT9	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
LASP1	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
LHX8	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
LMNA	Proximity Label-MS	physical	22412018, (Europe PMC)	NA	BioGRID
LYZ	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
MAGEC3	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
MAP4K1	Affinity Capture-Western, Reconstituted Complex, peptide array, pull down	physical, physical association	11279207, 17474147, 9788432, 9891069, (Europe PMC)	0.59	BioGRID, IntAct
MAP4K5	Affinity Capture-MS, Affinity Capture-Western, coimmunoprecipitation, pull down, tandem affinity purification	association, physical, physical association	10648385, 19380743, 9788432, (Europe PMC)	0.64	BioGRID, IntAct
MAPK4	Two-hybrid, two hybrid array	physical, physical association	21988832, (Europe PMC)	0.37	BioGRID, IntAct
MAPK8	Affinity Capture-Western, Co-localization, Reconstituted Complex, proximity ligation assay, pull down	physical, physical association	11432831, 16982329, 25241761, (Europe PMC)	0.59	BioGRID, IntAct, MINT
MET	Affinity Capture-Western, fluorescence polarization spectroscopy	direct interaction, physical	24728074, 24828152, (Europe PMC)	0.44	BioGRID, IntAct
MICAL1	Reconstituted Complex	physical	11827972, (Europe PMC)	NA	BioGRID
MNDA	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
MPDZ	Affinity Capture-MS, anti tag coimmunoprecipitation	association, physical	26496610, (Europe PMC)	0.35	BioGRID, IntAct
MPG	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
MRPL48	Affinity Capture-MS, anti tag coimmunoprecipitation	association, physical	26496610, (Europe PMC)	0.35	BioGRID, IntAct
MYLIP	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
MYOZ2	Two-hybrid	physical	25814554, (Europe PMC)	NA	BioGRID
NANS	Co-fractionation	physical	22939629, (Europe PMC)	NA	BioGRID
NCKIPSD	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
NEDD9	Affinity Capture-Western, Reconstituted Complex	physical	8879209, 9295052, 9497377, (Europe PMC)	NA	BioGRID
NUFIP2	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
NUMA1	Affinity Capture-MS	physical	26496610, (Europe PMC)	NA	BioGRID
ODF2	Proximity Label-MS, proximity-dependent biotin identification	association, physical	26638075, (Europe PMC)	0.35	BioGRID, IntAct
OFCC1	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
PAFAH1B2	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
PDGFRA	Affinity Capture-Western, Co-localization, Reconstituted Complex, coimmunoprecipitation, enzymatic study, proximity ligation assay, pull down	association, physical, physical association	10733900, 25241761, 9546424, (Europe PMC)	0.71	BioGRID, IntAct, MINT
PDGFRB	Affinity Capture-Western, Reconstituted Complex, pull down	association, physical	10733900, (Europe PMC)	0.35	BioGRID, IntAct, MINT
PEAK1	Affinity Capture-MS, anti tag coimmunoprecipitation, tandem affinity purification	association, physical	19380743, 20534451, (Europe PMC)	0.35	BioGRID, IntAct
PHC2	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
PIK3C2B	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
PIK3CA	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
PIK3CB	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
PIK3CD	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
PIK3R1	Affinity Capture-MS, Affinity Capture-Western, Co-localization, Two-hybrid, proximity ligation assay, tandem affinity purification, two hybrid array, validated two hybrid	association, physical, physical association	10962563, 11418612, 19380743, 25241761, 25814554, (Europe PMC)	0.49, 0.56	BioGRID, IntAct
PIK3R2	Affinity Capture-MS, Two-hybrid, tandem affinity purification, two hybrid array, validated two hybrid	association, physical, physical association	19380743, 25814554, (Europe PMC)	0.35, 0.49	BioGRID, IntAct
PIK3R3	Affinity Capture-MS, Two-hybrid, tandem affinity purification, two hybrid array, validated two hybrid	association, physical, physical association	19380743, 25814554, (Europe PMC)	0.49, 0.62	BioGRID, IntAct
PLSCR1	Two-hybrid, two hybrid pooling approach	physical, physical association	16189514, (Europe PMC)	0.37	BioGRID, IntAct
POT1	Two-hybrid, bimolecular fluorescence complementation	physical, physical association	21044950, (Europe PMC)	0.37	BioGRID, IntAct
PPFIBP2	Two-hybrid, two hybrid array, two hybrid pooling approach, two hybrid prey pooling approach, validated two hybrid	physical, physical association	19447967, 26871637, (Europe PMC)	0.67	BioGRID, IntAct
PPP1CA	Two-hybrid, two hybrid	physical, physical association	22321011, (Europe PMC)	0.37	BioGRID, IntAct
PPP1R12A	Affinity Capture-MS	physical	26496610, (Europe PMC)	NA	BioGRID
PRKACA	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
PRRC2B	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
PRRG2	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
PSMC1	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
PSMC6	Two-hybrid, two hybrid array, two hybrid pooling approach, two hybrid prey pooling approach, validated two hybrid	physical, physical association	16189514, 26871637, (Europe PMC)	0.76	BioGRID, IntAct
PTK2	Affinity Capture-Western, Reconstituted Complex, Two-hybrid, anti bait coimmunoprecipitation, two hybrid array, validated two hybrid	physical, physical association	10085298, 12615911, 25814554, 7561682, (Europe PMC)	0.63	BioGRID, IntAct
PTPN1	Reconstituted Complex	physical	8940134, (Europe PMC)	NA	BioGRID
PTPN11	Affinity Capture-MS, Affinity Capture-Western	physical	10464310, 19380743, (Europe PMC)	NA	BioGRID
PTPN4	Affinity Capture-Western, Two-hybrid, anti tag coimmunoprecipitation, confocal microscopy, two hybrid	colocalization, physical, physical association	21988832, (Europe PMC)	0.54	BioGRID, IntAct
PTTG1	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
PXN	Affinity Capture-Western, Reconstituted Complex, anti bait coimmunoprecipitation	physical, physical association	10085298, 12198159, 12799422, 26656091, (Europe PMC)	0.40	BioGRID, IntAct, MINT
RAB2B	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
RAPGEF1	Affinity Capture-MS, Affinity Capture-Western, Far Western, Reconstituted Complex, coimmunoprecipitation, peptide array, pull down, tandem affinity purification	association, physical, physical association	10464310, 17474147, 17515907, 18835194, 19380743, 7512734, 7531694, 8621483, 8943292, (Europe PMC)	0.35, 0.69	BioGRID, IntAct, MINT
RET	Co-localization, Reconstituted Complex, proximity ligation assay	physical, physical association	25241761, 9393871, (Europe PMC)	0.40	BioGRID, IntAct
RIN3	Two-hybrid, peptide array, two hybrid array	physical, physical association	17474147, 25814554, (Europe PMC)	0.57	BioGRID, IntAct
RNGTT	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
RPL23	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
RPS19	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
RPS25	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
RPS3	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
RPS5	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
RTCB	Two-hybrid	physical	25814554, (Europe PMC)	NA	BioGRID
RYBP	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
SAXO1	Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical, physical association	25416956, (Europe PMC)	0.56	BioGRID, IntAct
SEMA4D	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
SEPT6	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
SERPINB6	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
SERPINH1	Co-fractionation	physical	22939629, (Europe PMC)	NA	BioGRID
SETD9	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
SFN	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
SH2D2A	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
SH3BP1	Reconstituted Complex	physical	1379745, (Europe PMC)	NA	BioGRID
SH3KBP1	Affinity Capture-Western	physical	11071869, (Europe PMC)	NA	BioGRID
SHB	Affinity Capture-Western	physical	10964504, (Europe PMC)	NA	BioGRID
SHC1	Affinity Capture-MS, Affinity Capture-Western, proximity ligation assay, tandem affinity purification	association, physical, physical association	10464310, 19380743, 23397142, 9617486, (Europe PMC)	0.56	BioGRID, IntAct
SLC25A13	Affinity Capture-MS, anti tag coimmunoprecipitation	association, physical	26496610, (Europe PMC)	0.35	BioGRID, IntAct
SOCS1	Reconstituted Complex, Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	10022833, 25814554, (Europe PMC)	0.49	BioGRID, IntAct
SOCS6	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
SOS1	Affinity Capture-MS, Affinity Capture-Western, Co-localization, Reconstituted Complex, peptide array, proximity ligation assay, tandem affinity purification	association, physical, physical association	17474147, 19380743, 25241761, 8810325, 8943292, (Europe PMC)	0.69	BioGRID, IntAct
SOS2	Affinity Capture-MS, peptide array, tandem affinity purification	association, physical, physical association	17474147, 19380743, (Europe PMC)	0.56	BioGRID, IntAct
SPR	Co-fractionation	physical	22939629, (Europe PMC)	NA	BioGRID
SPRR2A	Reconstituted Complex, protein array	direct interaction, physical	18155796, (Europe PMC)	0.44	BioGRID, IntAct, MINT
SRC	Affinity Capture-Western, proximity ligation assay	physical, physical association	10962563, 12615911, 23397142, (Europe PMC)	0.40	BioGRID, IntAct
SRCIN1	Affinity Capture-Western	physical	14657239, (Europe PMC)	NA	BioGRID
SRM	Co-fractionation	physical	26344197, (Europe PMC)	NA	BioGRID
STAT4	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
STAT5A	Co-localization, proximity ligation assay	physical, physical association	25241761, (Europe PMC)	0.40	BioGRID, IntAct
STRN4	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
SYN1	Affinity Capture-Western	physical	10899172, (Europe PMC)	NA	BioGRID
TCAP	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
TDG	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
TERF2IP	Two-hybrid, bimolecular fluorescence complementation, pull down	physical, physical association	21044950, (Europe PMC)	0.51	BioGRID, IntAct
TM4SF19	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
TP53	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
TRIM15	Affinity Capture-Western, PCA	physical	25450970, (Europe PMC)	NA	BioGRID
TSG101	Affinity Capture-Western, Co-localization	physical	27764233, (Europe PMC)	NA	BioGRID
TUBA1A	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
TUBA1C	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
TUBA4B	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
TUBB	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
TUBB2A	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
TUBB3	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
TUBB4B	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
TWIST2	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
UBASH3A	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
UBASH3B	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
UBC	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
VAC14	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
WDR83	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
WEE1	Affinity Capture-Western	physical	11839808, (Europe PMC)	NA	BioGRID
XPO1	Two-hybrid	physical	11839808, (Europe PMC)	NA	BioGRID
YWHAG	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
YWHAQ	Affinity Capture-MS	physical	19380743, (Europe PMC)	NA	BioGRID
ZAP70	Reconstituted Complex, pull down	association, physical	10419455, 16339550, (Europe PMC)	0.35	BioGRID, IntAct, MINT
ZKSCAN7	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
ZNF557	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct

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Interacting partner	Detection method	Interaction type	PubMed IDs	Interaction Score	Source
ABI1	array technology	direct interaction	20598684, (Europe PMC)	0.44	IntAct, MINT
ABL1	Affinity Capture-MS, Affinity Capture-Western, Biochemical Activity, Reconstituted Complex, Two-hybrid, nuclear magnetic resonance, peptide array, protein kinase assay, pull down	association, phosphorylation reaction, physical, physical association	12097319, 12384576, 15696159, 17474147, 17515907, 18619508, 18835194, 19380743, 24412932, 8194526, 9747873, (Europe PMC)	0.35, 0.44, 0.56	BioGRID, IntAct, MINT
ABL2	Affinity Capture-MS, Biochemical Activity, Reconstituted Complex, Two-hybrid, peptide array, protein kinase assay, tandem affinity purification, two hybrid array, validated two hybrid	association, phosphorylation reaction, physical, physical association	15886098, 17474147, 19380743, 25814554, 8194526, (Europe PMC)	0.49, 0.70	BioGRID, IntAct
AFF2	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
AGO1	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
AGO2	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
AGPAT4	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
AIRE	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
AKAP2	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
AKAP6	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
ALS2CR12	two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical association	26871637, (Europe PMC)	0.56	IntAct
ANKZF1	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
ANLN	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
AP2A1	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
AP2M1	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
APOL5	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
AR	fluorescence polarization spectroscopy	direct interaction	24728074, (Europe PMC)	0.44	IntAct
ARHGEF11	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
ARPC1B	Affinity Capture-MS, anti tag coimmunoprecipitation	association, physical	26496610, (Europe PMC)	0.35	BioGRID, IntAct
ASAP1	Affinity Capture-MS, Reconstituted Complex, peptide array	physical, physical association	17474147, 19380743, 9819391, (Europe PMC)	0.40	BioGRID, IntAct
ASAP2	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
ASAP3	Affinity Capture-MS, bimolecular fluorescence complementation, tandem affinity purification, two hybrid array, two hybrid prey pooling approach, validated two hybrid	association, physical, physical association	19380743, 26871637, (Europe PMC)	0.70	BioGRID, IntAct
ASB9	Affinity Capture-Luminescence, Two-hybrid, luminescence based mammalian interactome mapping, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.51	BioGRID, IntAct
ASCL4	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
ATF3	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
ATXN1	Affinity Capture-Western, Two-hybrid, pull down, two hybrid array, two hybrid prey pooling approach	physical, physical association	16713569, 23275563, (Europe PMC)	0.63	BioGRID, IntAct
BATF3	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
BCAR1	Affinity Capture-MS, Affinity Capture-Western, Protein-peptide, Reconstituted Complex, anti bait coimmunoprecipitation, pull down	association, physical, physical association	10085298, 10329689, 10464310, 10747099, 10753828, 11278916, 11369773, 11839772, 12615911, 12799422, 14657239, 15492270, 15673687, 16267043, 17038317, 18835194, 19380743, 22974441, 9581808, 9920935, (Europe PMC)	0.76	BioGRID, IntAct, MINT
BEX5	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
BICRA	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
BMX	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
BRD4	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
BUB1	Two-hybrid, two hybrid pooling approach	physical, physical association	16189514, (Europe PMC)	0.37	BioGRID, IntAct
C1orf94	two hybrid array, two hybrid prey pooling approach, validated two hybrid	physical association	26871637, (Europe PMC)	0.56	IntAct
C4orf17	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
C6orf141	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
CASS4	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
CAST	Co-fractionation, peptide array	physical, physical association	17474147, 26344197, (Europe PMC)	0.40	BioGRID, IntAct
CBL	Affinity Capture-MS, Affinity Capture-Western, Protein-peptide, Reconstituted Complex, Two-hybrid, anti bait coimmunoprecipitation, coimmunoprecipitation, pull down, tandem affinity purification, two hybrid array, validated two hybrid	association, physical, physical association	10962563, 11792427, 12393469, 15581361, 16289966, 18835194, 19380743, 22974441, 25814554, 8524328, 8621483, 8626404, 8626543, 8662998, 8683103, 8943292, 9129019, 9178909, 9416834, 9461587, 9617486, 9988765, (Europe PMC)	0.49, 0.88	BioGRID, IntAct, MINT
CBLB	Affinity Capture-MS, Two-hybrid, tandem affinity purification, two hybrid array, validated two hybrid	association, physical, physical association	19380743, 25814554, (Europe PMC)	0.49, 0.62	BioGRID, IntAct
CBLC	Reconstituted Complex, Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	10362357, 25814554, (Europe PMC)	0.49	BioGRID, IntAct
CCP110	Proximity Label-MS, proximity-dependent biotin identification	association, physical	26638075, (Europe PMC)	0.35	BioGRID, IntAct
CD93	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
CDH11	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
CELSR2	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
CEP128	Proximity Label-MS, proximity-dependent biotin identification	association, physical	26638075, (Europe PMC)	0.35	BioGRID, IntAct
CEP19	Proximity Label-MS, proximity-dependent biotin identification	association, physical	26638075, (Europe PMC)	0.35	BioGRID, IntAct
CEP89	Proximity Label-MS, proximity-dependent biotin identification	association, physical	26638075, (Europe PMC)	0.35	BioGRID, IntAct
CHTF18	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
CLNK	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
CNDP2	Two-hybrid, two hybrid array, validated two hybrid	physical, physical association	25814554, (Europe PMC)	0.49	BioGRID, IntAct
CNTFR	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
CNTNAP1	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
CORO6	Two-hybrid, two hybrid array	physical, physical association	25814554, (Europe PMC)	0.37	BioGRID, IntAct
COX6C	Affinity Capture-MS, anti tag coimmunoprecipitation	association, physical	26496610, (Europe PMC)	0.35	BioGRID, IntAct
CSMD2	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
DDX41	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
DDX6	Affinity Capture-MS, tandem affinity purification	association, physical	19380743, (Europe PMC)	0.35	BioGRID, IntAct
DKKL1	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
DLGAP1	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
DLGAP2	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
DLGAP3	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
DLGAP4	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
DLX4	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
DNM2	peptide array	physical association	17474147, (Europe PMC)	0.40	IntAct
DOCK1	Affinity Capture-Western, Co-fractionation, Reconstituted Complex, peptide array, pull down	association, physical, physical association	10559471, 11369773, 12615911, 15673687, 17474147, 17515907, 26344197, 8657152, 8662907, (Europe PMC)	0.35, 0.40	BioGRID, IntAct
DOCK3	peptide array	physical association	17474147, (Europe PMC)

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