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Statistics
241 human active and 13 inactive phosphatases in total;
194 phosphatases have substrate data;
--------------------------------
336 protein substrates;
83 non-protein substrates;
1215 dephosphorylation interactions;
--------------------------------
299 KEGG pathways;
876 Reactome pathways;
--------------------------------
last scientific update:
11 Mar, 2019
last maintenance update:
01 Sep, 2023
194 phosphatases have substrate data;
--------------------------------
336 protein substrates;
83 non-protein substrates;
1215 dephosphorylation interactions;
--------------------------------
299 KEGG pathways;
876 Reactome pathways;
--------------------------------
last scientific update:
11 Mar, 2019
last maintenance update:
01 Sep, 2023
Top
STAM2
Gene Name  | STAM2 (QuickGO) | (A quick tutorial to explore the interctive visulaization) Representative structure: 2L0T |
| Synonyms | STAM2, HBP | |
| Protein Name | STAM2 | |
| Alternative Name(s) | Signal transducing adapter molecule 2;STAM-2;Hrs-binding protein; | |
| Protein Family | Belongs to the STAM family | |
| EntrezGene ID | 10254 (Comparitive Toxicogenomics) | |
| UniProt AC (Human) | O75886 (protein sequence) | |
| Enzyme Class | N/A | |
| Molecular Weight | 58164 Dalton | |
| Protein Length | 525 amino acids (AA) | |
| Genome Browsers | NCBI | ENSG00000115145 (Ensembl) | UCSC | |
| Crosslinking annotations | Query our ID-mapping table | |
| Orthologues | Quest For Orthologues (QFO) | GeneTree | eggNOG - KOG2199 | eggNOG - ENOG410XTSY | |
| Phosphorylation Network | Visualize | |
| Domain organization, Expression, Diseases | (show / hide) |
| Protein Domain | InterPro | Pfam | SMART | 3D Structure | PDB | DrugPort | ModBase | SwissModel |
| Polypeptide organization (Pfam)  | |||
| Gene Expression | Gene Expression Atlas | Disease | OMIM | DisGeNet | ClinVar |
| Protein-protein interactions | STRING | IntAct | MINT | BioGRID | ||
| Phosphosites | Phospho.ELM | PhosphoSitePlus |
| Localization, Function, Catalytic activity and Sequence | (show / hide) |
| Localization (UniProt annotation) | |||
| Cytoplasm Early endosomemembrane | |||
| Function (UniProt annotation) | |||
| Involved in intracellular signal transduction mediatedby cytokines and growth factors Upon IL-2 and GM-CSL stimulation,it plays a role in signaling leading to DNA synthesis and MYCinduction May also play a role in T-cell development Involved indown-regulation of receptor tyrosine kinase via multivesicularbody (MVBs) when complexed with HGS (ESCRT-0 complex) The ESCRT-0complex binds ubiquitin and acts as sorting machinery thatrecognizes ubiquitinated receptors and transfers them to furthersequential lysosomal sorting/trafficking processes (Bysimilarity) | |||
| Catalytic Activity (UniProt annotation) | |||
| N/A | |||
| Protein Sequence | |||
| MPLFTANPFEQDVEKATNEYNTTEDWSLIMDICDKVGSTPNGAKDCLKAIMKRVNHKVPHVALQALTLLGACVANCGKIF HLEVCSRDFATEVRAVIKNKAHPKVCEKLKSLMVEWSEEFQKDPQFSLISATIKSMKEEGITFPPAGSQTVSAAAKNGTS SNKNKEDEDIAKAIELSLQEQKQQHTETKSLYPSSEIQLNNKVARKVRALYDFEAVEDNELTFKHGEIIIVLDDSDANWW KGENHRGIGLFPSNFVTTNLNIETEAAAVDKLNVIDDDVEEIKKSEPEPVYIDEDKMDRALQVLQSIDPTDSKPDSQDLL DLEDICQQMGPMIDEKLEEIDRKHSELSELNVKVLEALELYNKLVNEAPVYSVYSKLHPPAHYPPASSGVPMQTYPVQSH GGNYMGQSIHQVTVAQSYSLGPDQIGPLRSLPPNVNSSVTAQPAQTSYLSTGQDTVSNPTYMNQNSNLQSATGTTAYTQQ MGMSVDMSSYQNTTSNLPQLAGFPVTVPAHPVAQQHTNYHQQPLL | |||
| Motif information from Eukaryotic Linear Motif atlas (ELM) | (show / hide) |
| ELM Motif | Motif Instance
| Description | Motif Regex |
| NA | NA | NA | NA |
| Gene Ontology (P: Process; F: Function and C: Component terms) | (show / hide) | |
| GO:0005654 | C:nucleoplasm |
| GO:0005737 | C:cytoplasm |
| GO:0005829 | C:cytosol |
| GO:0006886 | P:intracellular protein transport |
| GO:0016197 | P:endosomal transport |
| GO:0016236 | P:macroautophagy |
| GO:0031901 | C:early endosome membrane |
| GO:0033565 | C:ESCRT-0 complex |
| GO:0036258 | P:multivesicular body assembly |
| GO:0042059 | P:negative regulation of epidermal growth factor receptor signaling pathway |
| GO:0043231 | C:intracellular membrane-bounded organelle |
| GO:0061024 | P:membrane organization |
STAM2 is dephosphorylated by following phosphatases:
| Phosphatase Gene Name | Phosphatase UniProt_AC | DephosphoSite and sequence (+/-5) in STAM2 (O75886) | Bioassay Type | PubMed ID | View in Europe PMC | Reliability of the interaction |
| PTPN1 | P18031 | Tyr-291_EPEPVyIDEDK, | In vitro and in vivo | 20504764, | Europe PMC |  |
| Pathway ID | Pathway Name | Pathway Description (KEGG) |
| hsa04144 | Endocytosis | Endocytosis is a mechanism for cells to remove ligands, nutrients, and plasma membrane (PM) proteins, and lipids from the cell surface, bringing them into the cell interior. Transmembrane proteins entering through clathrin-dependent endocytosis (CDE) have sequences in their cytoplasmic domains that bind to the APs (adaptor-related protein complexes) and enable their rapid removal from the PM. In addition to APs and clathrin, there are numerous accessory proteins including dynamin. Depending on the various proteins that enter the endosome membrane, these cargoes are sorted to distinct destinations. Some cargoes, such as nutrient receptors, are recycled back to the PM. Ubiquitylated membrane proteins, such as activated growth-factor receptors, are sorted into intraluminal vesicles and eventually end up in the lysosome lumen via multivesicular endosomes (MVEs). There are distinct mechanisms of clathrin-independent endocytosis (CIE) depending upon the cargo and the cell type. |
| hsa04630 | Jak-STAT signaling pathway | The Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway is one of a handful of pleiotropic cascades used to transduce a multitude of signals for development and homeostasis in animals, from humans to flies. In mammals, the JAK/STAT pathway is the principal signaling mechanism for a wide array of cytokines and growth factors. Following the binding of cytokines to their cognate receptor, STATs are activated by members of the JAK family of tyrosine kinases. Once activated, they dimerize and translocate to the nucleus and modulate the expression of target genes. In addition to the activation of STATs, JAKs mediate the recruitment of other molecules such as the MAP kinases, PI3 kinase etc. These molecules process downstream signals via the Ras-Raf-MAP kinase and PI3 kinase pathways which results in the activation of additional transcription factors. |
| Pathway ID | Pathway Name | Pathway Description (KEGG) |
| R-HSA-182971 | EGFR downregulation. | Regulation of receptor tyrosine kinase (RTK) activity is implicated in the control of almost all cellular functions. One of the best understood RTKs is epidermal growth factor receptor (EGFR). Growth factors can bind to EGFR and activate it to initiate signalling cascades within the cell. EGFRs can also be recruited to clathrin-coated pits which can be internalised into endocytic vesicles. From here, EGFRs can either be recycled back to the plasma membrane or directed to lysosomes for destruction.This provides a mechanism by which EGFR signalling is negatively regulated and controls the strength and duration of EGFR-induced signals. It also prevents EGFR hyperactivation as commonly seen in tumorigenesis.The proto-oncogene Cbl can negatively regulate EGFR signalling. The Cbl family of RING-type ubiquitin ligases are able to poly-ubiquitinate EGFR, an essential step in EGFR degradation. All Cbl proteins have a unique domain that recognises phosphorylated tyrosine residues on activated EGFRs. They also direct the ubiquitination and degradation of activated EGFRs by recruiting ubiquitin-conjugation enzymes. Cbl proteins function by specifically targeting activated EGFRs and mediating their down-regulation, thus providing a means by which signaling processes can be negatively regulated.Cbl also promotes receptor internalization via it's interaction with an adaptor protein, CIN85 (Cbl-interacting protein of 85kDa). CIN85 binds to Cbl via it's SH3 domain and is enhanced by the EGFR-induced tyrosine phosphorylation of Cbl. The proline-rich region of CIN85 interacts with endophilins which are regulatory components of clathrin-coated vesicles (CCVs). Endophilins bind to membranes and induce membrane curvature, in conjunction with other proteins involved in CCV formation. The rapid recruitment of endophilin to the activated receptor complex by CIN85 is the mechanism which controls receptor internalization |
| R-HSA-5689880 | Ub-specific processing proteases. | Ub-specific processing proteases (USPs) are the largest of the DUB families with more than 50 members in humans. The USP catalytic domain varies considerably in size and consists of six conserved motifs with N- or C-terminal extensions and insertions occurring between the conserved motifs (Ye et al. 2009). Two highly conserved regions comprise the catalytic triad, the Cys-box (Cys) and His-box (His and Asp/Asn) (Nijman et al. 2005, Ye et al. 2009, Reyes-Turcu & Wilkinson 2009). They recognize their substrates by interactions of the variable regions with the substrate protein directly, or via scaffolds or adapters in multiprotein complexes |
| R-HSA-6807004 | Negative regulation of MET activity. | Signaling by MET receptor is negatively regulated mainly by MET receptor dephosphorylation or MET receptor degradation. Protein tyrosine phosphatase PTPRJ dephosphorylates MET tyrosine residue Y1349, thus removing the docking site for the GAB1 adapter (Palka et al. 2003). Protein tyrosine phosphatases PTPN1 and PTPN2 dephosphorylate MET tyrosines Y1234 and Y1235 in the kinase activation loop, thus attenuating catalytic activity of MET (Sangwan et al. 2008). The E3 ubiquitin ligase CBL promotes ubiquitination of the activated MET receptor and subsequent MET degradation. CBL contains a RING finger domain that engages E2 protein ubiquitin ligases to mediate ubiquitination of MET, which may occur at the cell membrane or in the early endocytic compartment. Ubiquitinated MET is degraded in a late endosomal or lysosomal compartment in a proteasome-dependent manner. The involvement of proteasome in MET degradation seems to be indirect, through an effect on MET endocytic trafficking (Jeffers et al. 1997, Peschard et al. 2001, Hammond et al. 2001, Petrelli et al. 2002). LRIG1 promotes lysosome-dependent degradation of MET in the absence of HGF-mediated activation (Lee et al. 2014, Oh et al. 2014).MET-mediated activation of RAS signaling is inhibited by MET receptor binding to MUC20 (Higuchi et al. 2004) or RANBP10 (Wang et al. 2004) |
| R-HSA-8856825 | Cargo recognition for clathrin-mediated endocytosis. | Recruitment of plasma membrane-localized cargo into clathrin-coated endocytic vesicles is mediated by interaction with a variety of clathrin-interacting proteins collectively called CLASPs (clathrin-associated sorting proteins). CLASP proteins, which may be monomeric or tetrameric, are recruited to the plasma membrane through interaction with phosphoinsitides and recognize linear or conformational sequences or post-translational modifications in the cytoplasmic tails of the cargo protein. Through bivalent interactions with clathrin and/or other CLASP proteins, they bridge the recruitment of the cargo to the emerging clathrin coated pit (reviewed in Traub and Bonifacino, 2013). The tetrameric AP-2 complex, first identified in early studies of clathrin-mediated endocytosis, was at one time thought to be the primary CLASP protein involved in cargo recognition at the plasma membrane, and indeed plays a key role in the endocytosis of cargo carrying dileucine- or tyrosine-based motifs. A number of studies have been performed to test whether AP-2 is essential for all forms of clathrin-mediated endocytosis (Keyel et al, 2006; Motely et al, 2003; Huang et al, 2004; Boucrot et al, 2010; Henne et al, 2010; Johannessen et al, 2006; Gu et al, 2013; reviewed in Traub, 2009; McMahon and Boucrot, 2011). Although depletion of AP-2 differentially affects the endocytosis of different cargo, extensive depletion of AP-2 through RNAi reduces clathrin-coated pit formation by 80-90%, and the CCPs that do form still contain AP-2, highlighting the critcical role of this complex in CME (Johannessen et al, 2006; Boucrot et al, 2010; Henne et al, 2010).In addition to AP-2, a wide range of other CLASPs including proteins of the beta-arrestin, stonin and epsin families, engage sorting motifs in other cargo and interact either with clathrin, AP-2 or each other to facilitate assembly of a clathin-coated pit (reviewed in Traub and Bonifacino, 2013) |
| R-HSA-8856828 | Clathrin-mediated endocytosis. | Clathrin-mediated endocytosis (CME) is one of a number of process that control the uptake of material from the plasma membrane, and leads to the formation of clathrin-coated vesicles (Pearse et al, 1975; reviewed in Robinson, 2015; McMahon and Boucrot, 2011; Kirchhausen et al, 2014). CME contributes to signal transduction by regulating the cell surface expression and signaling of receptor tyrosine kinases (RTKs) and G-protein coupled receptors (GPCRs). Most RTKs exhibit a robust increase in internalization rate after binding specific ligands; however, some RTKs may also exhibit significant ligand-independent internalization (reviewed in Goh and Sorkin, 2013). CME controls RTK and GPCR signaling by organizing signaling both within the plasma membrane and on endosomes (reviewed in Eichel et al, 2016; Garay et al, 2015; Vieira et al, 1996; Sorkin and von Zastrow, 2014; Di Fiori and von Zastrow, 2014; Barbieri et al, 2016). CME also contributes to the uptake of material such as metabolites, hormones and other proteins from the extracellular space, and regulates membrane composition by recycling membrane components and/or targeting them for degradation. Clathrin-mediated endocytosis involves initiation of clathrin-coated pit (CCP) formation, cargo selection, coat assembly and stabilization, membrane scission and vesicle uncoating. Although for simplicity in this pathway, the steps leading to a mature CCP are represented in a linear and temporally distinct fashion, the formation of a clathrin-coated vesicle is a highly heterogeneous process and clear temporal boundaries between these processes may not exist (see for instance Taylor et al, 2011; Antonescu et al, 2011; reviewed in Kirchhausen et al, 2014). Cargo selection in particular is a critical aspect of the formation of a mature and stable CCP, and many of the proteins involved in the initiation and maturation of a CCP contribute to cargo selection and are themselves stabilized upon incorporation of cargo into the nascent vesicle (reviewed in Kirchhausen et al, 2014; McMahon and Boucrot, 2011).Although the clathrin triskelion was identified early as a major component of the coated vesicles, clathrin does not bind directly to membranes or to the endocytosed cargo. Vesicle formation instead relies on many proteins and adaptors that can bind the plasma membrane and interact with cargo molecules. Cargo selection depends on the recognition of endocytic signals in cytoplasmic tails of the cargo proteins by adaptors that interact with components of the vesicle's inner coat. The classic adaptor for clathrin-coated vesicles is the tetrameric AP-2 complex, which along with clathrin was identified early as a major component of the coat. Some cargo indeed bind directly to AP-2, but subsequent work has revealed a large family of proteins collectively known as CLASPs (clathrin- associated sorting proteins) that mediate the recruitment of diverse cargo into the emerging clathrin-coated vesicles (reviewed in Traub and Bonifacino, 2013). Many of these CLASP proteins themselves interact with AP-2 and clathrin, coordinating cargo recruitment with coat formation (Schmid et al, 2006; Edeling et al, 2006; reviewed in Traub and Bonifacino, 2013; Kirchhausen et al, 2014). Initiation of CCP formation is also influenced by lipid composition, regulated by clathrin-associated phosphatases and kinases (reviewed in Picas et al, 2016). The plasma membrane is enriched in PI(4,5)P2. Many of the proteins involved in initiating clathrin-coated pit formation bind to PI(4,5)P2 and induce membrane curvature through their BAR domains (reviewed in McMahon and Boucrot, 2011; Daumke et al, 2014). Epsin also contributes to early membrane curvature through its Epsin N-terminal homology (ENTH) domain, which promotes membrane curvature by inserting into the lipid bilayer (Ford et al, 2002). Following initiation, some CCPs progress to formation of vesicles, while others undergo disassembly at the cell surface without producing vesicles (Ehrlich et al, 2004; Loerke et al, 2009; Loerke et al, 2011; Aguet et al, 2013; Taylor et al, 2011). The assembly and stabilization of nascent CCPs is regulated by several proteins and lipids (Mettlen et al, 2009; Antonescu et al, 2011).Maturation of the emerging clathrin-coated vesicle is accompanied by further changes in the lipid composition of the membrane and increased membrane curvature, promoted by the recruitment of N-BAR domain containing proteins (reviewed in Daumke et al, 2014; Ferguson and De Camilli, 2012; Picas et al, 2016). Some N-BAR domain containing proteins also contribute to the recruitment of the large GTPase dynamin, which is responsible for scission of the mature vesicle from the plasma membrane (Koh et al, 2007; Lundmark and Carlsson, 2003; Soulet et al, 2005; David et al, 1996; Owen et al, 1998; Shupliakov et al, 1997; Taylor et al, 2011; Ferguson et al, 2009; Aguet et al, 2013; Posor et al, 2013; Chappie et al, 2010; Shnyrova et al, 2013; reviewed in Mettlen et al, 2009; Daumke et al, 2014). After vesicle scission, the clathrin coat is dissociated from the new vesicle by the ATPase HSPA8 (also known as HSC70) and its DNAJ cofactor auxilin, priming the vesicle for fusion with a subsequent endocytic compartment and releasing clathrin for reuse (reviewed in McMahon and Boucrot, 2011; Sousa and Laufer, 2015) |
| R-HSA-8875360 | InlB-mediated entry of Listeria monocytogenes into host cell. | InlB, a cell wall protein of Listeria monocytogenes, binds MET receptor, acting as an HGF agonist (Shen et al. 2000, Veiga and Cossart 2005). Listeria monocytogenes InlB proteins dimerize through their leucine-rich repeat regions (LRRs), promoting dimerization of MET receptors that they are bound to (Ferraris et al. 2010). InlB-induced MET receptor dimerization is followed by MET trans-autophosphorylation and activation of downstream RAS/RAF/MAPK signaling and PI3K/AKT signaling (Niemann et al. 2007, Ferraris et al. 2010). InlB-bound phosphorylated MET receptor recruits the E3 ubiquitin ligase CBL through GRB2. CBL-mediated monoubiquitination of InlB-bound MET promotes endocytosis and entry of Listeria monocytogenes to host cells (Veiga and Cossart 2005). CIN85 is necessary for endocytosis-mediated entry of Listeria monocytogenes triggered by CBL-mediated monoubiquitination of MET (Veiga and Cossart 2005). Proteins involved in clathrin-mediated endocytosis EPS15 and HGS (Hrs) are both necessary for CBL and MET-mediated entry of Listeria monocytogenes into host cells (Veiga and Cossart 2005).A potential coreceptor role of CD44 in InlB-mediated MET activation is contradictory (Jung et al. 2009, Dortet et al. 2010) |
| R-HSA-917729 | Endosomal Sorting Complex Required For Transport (ESCRT). | Many plasma membrane proteins are in a constant flux throughout the internal trafficking pathways of the cell. Some receptors are continuously internalized into recycling endosomes and returned to the cell surface. Others are sorted into intralumenal vesicles of morphologically distinctive endosomes that are known as multivesicular bodies (MVBs). These MVBs fuse with lysosomes, resulting in degradation of their cargo by lysosomal acidic hydrolases.\n\nEndosomes can be operationally defined as being either early or late, referring to the relative time it takes for endocytosed material to reach either stage. Ultrastructural studies indicate that early endosomes are predominantly tubulovesicular structures, which constitute a major sorting platform in the cell, whereas late endosomes show the characteristics of typical MVBs and are capable of fusing with lysosomes.\n\nA well characterized signal for shunting membrane proteins into the degradative MVB pathway is the ubiquitylation of these cargoes. At the center of a vast protein:protein and protein:lipid interaction network that underpins ubiquitin mediated sorting to the lysosome are the endosomal sorting complexes required for transport (ESCRTs), which are conserved throughout all major eukaryotic taxa |
| Interacting partner | Detection method | Interaction type | PubMed IDs | Interaction Score | Source |
| ACTN1 | Co-fractionation | physical | 22863883, (Europe PMC) | NA | BioGRID |
| ARRDC3 | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.70 | BioGRID, IntAct |
| ATXN1 | Two-hybrid, two hybrid prey pooling approach | physical, physical association | 23275563, (Europe PMC) | 0.37 | BioGRID, IntAct |
| CBX5 | Protein-peptide | physical | 15882967, (Europe PMC) | NA | BioGRID |
| COG7 | Co-fractionation | physical | 26344197, (Europe PMC) | NA | BioGRID |
| DAZAP2 | Two-hybrid, two hybrid array, two hybrid pooling approach, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 16189514, 25416956, (Europe PMC) | 0.76 | BioGRID, IntAct |
| DCAF8 | Co-fractionation | physical | 22863883, (Europe PMC) | NA | BioGRID |
| EGFR | Affinity Capture-Western, Reconstituted Complex, anti tag coimmunoprecipitation, confocal microscopy | colocalization, physical, physical association | 15212941, 17403676, (Europe PMC) | 0.46 | BioGRID, IntAct |
| EPS15 | Affinity Capture-Western | physical | 12551915, (Europe PMC) | NA | BioGRID |
| FAM189A2 | Two-hybrid, two hybrid | physical, physical association | 21516116, 25416956, (Europe PMC) | 0.37 | BioGRID, IntAct, MINT |
| FBF1 | Proximity Label-MS, proximity-dependent biotin identification | association, physical | 26638075, (Europe PMC) | 0.35 | BioGRID, IntAct |
| HGS | Affinity Capture-Western, Co-fractionation, Co-localization, Two-hybrid, two hybrid pooling approach | physical, physical association | 12551915, 13679051, 15113837, 16189514, 16429130, 20504764, 22939629, 26344197, (Europe PMC) | 0.37 | BioGRID, IntAct |
| ITCH | Reconstituted Complex | physical | 22275353, (Europe PMC) | NA | BioGRID |
| JAK1 | Affinity Capture-Western, Biochemical Activity, anti tag coimmunoprecipitation, experimental interaction detection | phosphorylation reaction, physical | 10899310, 10993906, (Europe PMC) | 0.50 | BioGRID, IntAct, MINT |
| JAK2 | Affinity Capture-Western | physical | 10899310, (Europe PMC) | NA | BioGRID |
| JAK3 | Affinity Capture-Western | physical | 10899310, (Europe PMC) | NA | BioGRID |
| KIF15 | Co-fractionation | physical | 22863883, (Europe PMC) | NA | BioGRID |
| KLHL42 | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.70 | BioGRID, IntAct |
| KRT8 | Co-fractionation | physical | 22939629, (Europe PMC) | NA | BioGRID |
| L3MBTL2 | Two-hybrid, two hybrid array, two hybrid pooling approach, two hybrid prey pooling approach | physical, physical association | 16189514, (Europe PMC) | 0.62 | BioGRID, IntAct |
| LCP2 | Two-hybrid, peptide array, two hybrid array, two hybrid prey pooling approach, validated two hybrid | direct interaction, physical, physical association | 17235283, 25416956, (Europe PMC) | 0.77 | BioGRID, IntAct, MINT |
| LITAF | Two-hybrid, two hybrid array, two hybrid bait and prey pooling approach, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, 25910212, (Europe PMC) | 0.79 | BioGRID, IntAct |
| MAP3K1 | Biochemical Activity | physical | 25260751, (Europe PMC) | NA | BioGRID |
| NAPA | Co-fractionation | physical | 22939629, (Europe PMC) | NA | BioGRID |
| OFD1 | Proximity Label-MS, proximity-dependent biotin identification | association, physical | 26638075, (Europe PMC) | 0.35 | BioGRID, IntAct |
| PHF10 | Two-hybrid, two hybrid array | physical, physical association | 21988832, (Europe PMC) | 0.37 | BioGRID, IntAct |
| PLEKHB2 | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.70 | BioGRID, IntAct |
| PPP1R13B | Co-fractionation | physical | 26344197, (Europe PMC) | NA | BioGRID |
| PTPN1 | Affinity Capture-Western, Reconstituted Complex | physical | 20504764, (Europe PMC) | NA | BioGRID |
| PTPN23 | Affinity Capture-Western | physical | 23477725, (Europe PMC) | NA | BioGRID |
| PTPRN2 | Proximity Label-MS | physical | 27880917, (Europe PMC) | NA | BioGRID |
| RIN1 | Affinity Capture-Western, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, confocal microscopy | colocalization, physical, physical association | 17403676, 22976291, (Europe PMC) | 0.56 | BioGRID, IntAct |
| RNF11 | Affinity Capture-Western, Reconstituted Complex, Two-hybrid, two hybrid | physical, physical association | 15231748, 23222715, 24377927, (Europe PMC) | 0.37 | BioGRID, IntAct, MINT |
| SEC13 | Affinity Capture-Western | physical | 19054391, (Europe PMC) | NA | BioGRID |
| SEC31A | Affinity Capture-Western | physical | 19054391, (Europe PMC) | NA | BioGRID |
| SF3A3 | Co-fractionation | physical | 22863883, (Europe PMC) | NA | BioGRID |
| SH2D4A | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.56 | BioGRID, IntAct |
| SRP19 | Co-fractionation | physical | 22863883, (Europe PMC) | NA | BioGRID |
| STAM | Co-localization | physical | 13679051, (Europe PMC) | NA | BioGRID |
| STAMBP | Affinity Capture-Western, Co-crystal Structure, Reconstituted Complex, Two-hybrid, two hybrid, two hybrid array, two hybrid pooling approach | physical, physical association | 16189514, 16730941, 19060904, 20159979, 27725184, (Europe PMC) | 0.71 | BioGRID, IntAct |
| TIMM8A | Two-hybrid, two hybrid array, two hybrid bait and prey pooling approach, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, 25910212, (Europe PMC) | 0.79 | BioGRID, IntAct |
| TMEM17 | Proximity Label-MS, proximity-dependent biotin identification | association, physical | 26638075, (Europe PMC) | 0.35 | BioGRID, IntAct |
| TMEM216 | Proximity Label-MS, proximity-dependent biotin identification | association, physical | 26638075, (Europe PMC) | 0.35 | BioGRID, IntAct |
| TP53 | Synthetic Growth Defect | genetic | 23284306, (Europe PMC) | NA | BioGRID |
| TP53BP2 | Co-fractionation | physical | 26344197, (Europe PMC) | NA | BioGRID |
| TPTE | Proximity Label-MS | physical | 27880917, (Europe PMC) | NA | BioGRID |
| TSG101 | Co-fractionation | physical | 26344197, (Europe PMC) | NA | BioGRID |
| TYK2 | Affinity Capture-Western | physical | 10899310, (Europe PMC) | NA | BioGRID |
| UBC | Co-crystal Structure, Reconstituted Complex, surface plasmon resonance | direct interaction, physical | 12750381, 20504764, 21121635, 22841719, 26601948, 27725184, 28190767, (Europe PMC) | 0.44 | BioGRID, IntAct, MINT |
| UBE2D1 | Biochemical Activity | physical | 17588522, (Europe PMC) | NA | BioGRID |
| UBE2D2 | Biochemical Activity | physical | 17588522, (Europe PMC) | NA | BioGRID |
| UBE2D3 | Biochemical Activity | physical | 17588522, (Europe PMC) | NA | BioGRID |
| UBE2H | Biochemical Activity | physical | 17588522, (Europe PMC) | NA | BioGRID |
| UBQLN1 | Affinity Capture-Western, two hybrid array, two hybrid prey pooling approach | physical, physical association | 16159959, (Europe PMC) | 0.49 | BioGRID, IntAct |
| UBQLN4 | Two-hybrid, two hybrid array | physical, physical association | 16713569, (Europe PMC) | 0.37 | BioGRID, IntAct |
| USP8 | Affinity Capture-Western, PCA, Protein-peptide, Reconstituted Complex | physical | 10982817, 13129930, 20736164, (Europe PMC) | NA | BioGRID |
| VCP | Co-fractionation | physical | 22863883, (Europe PMC) | NA | BioGRID |
| VPS37C | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.70 | BioGRID, IntAct |
| XPO1 | Affinity Capture-MS | physical | 26673895, (Europe PMC) | NA | BioGRID |
| XRN1 | Two-hybrid, two hybrid | physical, physical association | 15231747, (Europe PMC) | 0.37 | BioGRID, IntAct |
| ZFYVE9 | Affinity Capture-Western | physical | 23222715, (Europe PMC) | NA | BioGRID |
| Interacting partner | Detection method | Interaction type | PubMed IDs | Interaction Score | Source |
| ARRDC3 | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.70 | BioGRID, IntAct |
| ATXN1 | Two-hybrid, two hybrid prey pooling approach | physical, physical association | 23275563, (Europe PMC) | 0.37 | BioGRID, IntAct |
| BET1 | proximity-dependent biotin identification | association | 29568061, (Europe PMC) | 0.35 | IntAct |
| DAZAP2 | Two-hybrid, two hybrid array, two hybrid pooling approach, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 16189514, 25416956, (Europe PMC) | 0.76 | BioGRID, IntAct |
| EGFR | Affinity Capture-Western, Reconstituted Complex, anti tag coimmunoprecipitation, confocal microscopy | colocalization, physical, physical association | 15212941, 17403676, (Europe PMC) | 0.46 | BioGRID, IntAct |
| FAM189A2 | Two-hybrid, two hybrid | physical, physical association | 21516116, 25416956, (Europe PMC) | 0.37 | BioGRID, IntAct, MINT |
| FBF1 | Proximity Label-MS, proximity-dependent biotin identification | association, physical | 26638075, (Europe PMC) | 0.35 | BioGRID, IntAct |
| HGS | Affinity Capture-Western, Co-fractionation, Co-localization, Two-hybrid, two hybrid pooling approach | physical, physical association | 12551915, 13679051, 15113837, 16189514, 16429130, 20504764, 22939629, 26344197, (Europe PMC) | 0.37 | BioGRID, IntAct |
| JAK1 | Affinity Capture-Western, Biochemical Activity, anti tag coimmunoprecipitation, experimental interaction detection | phosphorylation reaction, physical | 10899310, 10993906, (Europe PMC) | 0.50 | BioGRID, IntAct, MINT |
| KLHL42 | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.70 | BioGRID, IntAct |
| L3MBTL2 | Two-hybrid, two hybrid array, two hybrid pooling approach, two hybrid prey pooling approach | physical, physical association | 16189514, (Europe PMC) | 0.62 | BioGRID, IntAct |
| LAMP1 | proximity-dependent biotin identification | association | 29568061, (Europe PMC) | 0.35 | IntAct |
| LCP2 | Two-hybrid, peptide array, two hybrid array, two hybrid prey pooling approach, validated two hybrid | direct interaction, physical, physical association | 17235283, 25416956, (Europe PMC) | 0.77 | BioGRID, IntAct, MINT |
| LITAF | Two-hybrid, two hybrid array, two hybrid bait and prey pooling approach, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, 25910212, (Europe PMC) | 0.79 | BioGRID, IntAct |
| OFD1 | Proximity Label-MS, proximity-dependent biotin identification | association, physical | 26638075, (Europe PMC) | 0.35 | BioGRID, IntAct |
| PHF10 | Two-hybrid, two hybrid array | physical, physical association | 21988832, (Europe PMC) | 0.37 | BioGRID, IntAct |
| PLEKHB2 | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.70 | BioGRID, IntAct |
| RIN1 | Affinity Capture-Western, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, confocal microscopy | colocalization, physical, physical association | 17403676, 22976291, (Europe PMC) | 0.56 | BioGRID, IntAct |
| RNF11 | Affinity Capture-Western, Reconstituted Complex, Two-hybrid, two hybrid | physical, physical association | 15231748, 23222715, 24377927, (Europe PMC) | 0.37 | BioGRID, IntAct, MINT |
| SH2D4A | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.56 | BioGRID, IntAct |
| STAMBP | Affinity Capture-Western, Co-crystal Structure, Reconstituted Complex, Two-hybrid, two hybrid, two hybrid array, two hybrid pooling approach | physical, physical association | 16189514, 16730941, 19060904, 20159979, 27725184, (Europe PMC) | 0.71 | BioGRID, IntAct |
| TGOLN2 | proximity-dependent biotin identification | association | 29568061, (Europe PMC) | 0.35 | IntAct |
| TIMM8A | Two-hybrid, two hybrid array, two hybrid bait and prey pooling approach, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, 25910212, (Europe PMC) | 0.79 | BioGRID, IntAct |
| TMEM17 | Proximity Label-MS, proximity-dependent biotin identification | association, physical | 26638075, (Europe PMC) | 0.35 | BioGRID, IntAct |
| TMEM216 | Proximity Label-MS, proximity-dependent biotin identification | association, physical | 26638075, (Europe PMC) | 0.35 | BioGRID, IntAct |
| UBC | Co-crystal Structure, Reconstituted Complex, surface plasmon resonance | direct interaction, physical | 12750381, 20504764, 21121635, 22841719, 26601948, 27725184, 28190767, (Europe PMC) | 0.44 | BioGRID, IntAct, MINT |
| UBQLN1 | Affinity Capture-Western, two hybrid array, two hybrid prey pooling approach | physical, physical association | 16159959, (Europe PMC) | 0.49 | BioGRID, IntAct |
| UBQLN4 | Two-hybrid, two hybrid array | physical, physical association | 16713569, (Europe PMC) | 0.37 | BioGRID, IntAct |
| VPS37C | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.70 | BioGRID, IntAct |
| XRN1 | Two-hybrid, two hybrid | physical, physical association | 15231747, (Europe PMC) | 0.37 | BioGRID, IntAct |
| Interacting partner | Detection method | Interaction type | PubMed IDs | Interaction Score | Source |
| JAK1 | Affinity Capture-Western, Biochemical Activity, anti tag coimmunoprecipitation, experimental interaction detection | phosphorylation reaction, physical | 10899310, 10993906, (Europe PMC) | 0.50 | BioGRID, IntAct, MINT |
| LCP2 | Two-hybrid, peptide array, two hybrid array, two hybrid prey pooling approach, validated two hybrid | direct interaction, physical, physical association | 17235283, 25416956, (Europe PMC) | 0.77 | BioGRID, IntAct, MINT |
| RNF11 | Affinity Capture-Western, Reconstituted Complex, Two-hybrid, two hybrid | physical, physical association | 15231748, 23222715, 24377927, (Europe PMC) | 0.37 | BioGRID, IntAct, MINT |
| UBC | Co-crystal Structure, Reconstituted Complex, surface plasmon resonance | direct interaction, physical | 12750381, 20504764, 21121635, 22841719, 26601948, 27725184, 28190767, (Europe PMC) | 0.44 | BioGRID, IntAct, MINT |
| Interacting partner | Detection method | Interaction type | PubMed IDs | Interaction Score | Source |
| ACTN1 | Co-fractionation | physical | 22863883, (Europe PMC) | NA | BioGRID |
| ARRDC3 | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.70 | BioGRID, IntAct |
| ATXN1 | Two-hybrid, two hybrid prey pooling approach | physical, physical association | 23275563, (Europe PMC) | 0.37 | BioGRID, IntAct |
| BET1 | proximity-dependent biotin identification | association | 29568061, (Europe PMC) | 0.35 | IntAct |
| CBX5 | Protein-peptide | physical | 15882967, (Europe PMC) | NA | BioGRID |
| COG7 | Co-fractionation | physical | 26344197, (Europe PMC) | NA | BioGRID |
| DAZAP2 | Two-hybrid, two hybrid array, two hybrid pooling approach, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 16189514, 25416956, (Europe PMC) | 0.76 | BioGRID, IntAct |
| DCAF8 | Co-fractionation | physical | 22863883, (Europe PMC) | NA | BioGRID |
| EGFR | Affinity Capture-Western, Reconstituted Complex, anti tag coimmunoprecipitation, confocal microscopy | colocalization, physical, physical association | 15212941, 17403676, (Europe PMC) | 0.46 | BioGRID, IntAct |
| EPS15 | Affinity Capture-Western | physical | 12551915, (Europe PMC) | NA | BioGRID |
| FAM189A2 | Two-hybrid, two hybrid | physical, physical association | 21516116, 25416956, (Europe PMC) | 0.37 | BioGRID, IntAct, MINT |
| FBF1 | Proximity Label-MS, proximity-dependent biotin identification | association, physical | 26638075, (Europe PMC) | 0.35 | BioGRID, IntAct |
| HGS | Affinity Capture-Western, Co-fractionation, Co-localization, Two-hybrid, two hybrid pooling approach | physical, physical association | 12551915, 13679051, 15113837, 16189514, 16429130, 20504764, 22939629, 26344197, (Europe PMC) | 0.37 | BioGRID, IntAct |
| ITCH | Reconstituted Complex | physical | 22275353, (Europe PMC) | NA | BioGRID |
| JAK1 | Affinity Capture-Western, Biochemical Activity, anti tag coimmunoprecipitation, experimental interaction detection | phosphorylation reaction, physical | 10899310, 10993906, (Europe PMC) | 0.50 | BioGRID, IntAct, MINT |
| JAK2 | Affinity Capture-Western | physical | 10899310, (Europe PMC) | NA | BioGRID |
| JAK3 | Affinity Capture-Western | physical | 10899310, (Europe PMC) | NA | BioGRID |
| KIF15 | Co-fractionation | physical | 22863883, (Europe PMC) | NA | BioGRID |
| KLHL42 | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.70 | BioGRID, IntAct |
| KRT8 | Co-fractionation | physical | 22939629, (Europe PMC) | NA | BioGRID |
| L3MBTL2 | Two-hybrid, two hybrid array, two hybrid pooling approach, two hybrid prey pooling approach | physical, physical association | 16189514, (Europe PMC) | 0.62 | BioGRID, IntAct |
| LAMP1 | proximity-dependent biotin identification | association | 29568061, (Europe PMC) | 0.35 | IntAct |
| LCP2 | Two-hybrid, peptide array, two hybrid array, two hybrid prey pooling approach, validated two hybrid | direct interaction, physical, physical association | 17235283, 25416956, (Europe PMC) | 0.77 | BioGRID, IntAct, MINT |
| LITAF | Two-hybrid, two hybrid array, two hybrid bait and prey pooling approach, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, 25910212, (Europe PMC) | 0.79 | BioGRID, IntAct |
| MAP3K1 | Biochemical Activity | physical | 25260751, (Europe PMC) | NA | BioGRID |
| NAPA | Co-fractionation | physical | 22939629, (Europe PMC) | NA | BioGRID |
| OFD1 | Proximity Label-MS, proximity-dependent biotin identification | association, physical | 26638075, (Europe PMC) | 0.35 | BioGRID, IntAct |
| PHF10 | Two-hybrid, two hybrid array | physical, physical association | 21988832, (Europe PMC) | 0.37 | BioGRID, IntAct |
| PLEKHB2 | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.70 | BioGRID, IntAct |
| PPP1R13B | Co-fractionation | physical | 26344197, (Europe PMC) | NA | BioGRID |
| PTPN1 | Affinity Capture-Western, Reconstituted Complex | physical | 20504764, (Europe PMC) | NA | BioGRID |
| PTPN23 | Affinity Capture-Western | physical | 23477725, (Europe PMC) | NA | BioGRID |
| PTPRN2 | Proximity Label-MS | physical | 27880917, (Europe PMC) | NA | BioGRID |
| RIN1 | Affinity Capture-Western, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, confocal microscopy | colocalization, physical, physical association | 17403676, 22976291, (Europe PMC) | 0.56 | BioGRID, IntAct |
| RNF11 | Affinity Capture-Western, Reconstituted Complex, Two-hybrid, two hybrid | physical, physical association | 15231748, 23222715, 24377927, (Europe PMC) | 0.37 | BioGRID, IntAct, MINT |
| SEC13 | Affinity Capture-Western | physical | 19054391, (Europe PMC) | NA | BioGRID |
| SEC31A | Affinity Capture-Western | physical | 19054391, (Europe PMC) | NA | BioGRID |
| SF3A3 | Co-fractionation | physical | 22863883, (Europe PMC) | NA | BioGRID |
| SH2D4A | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.56 | BioGRID, IntAct |
| SRP19 | Co-fractionation | physical | 22863883, (Europe PMC) | NA | BioGRID |
| STAM | Co-localization | physical | 13679051, (Europe PMC) | NA | BioGRID |
| STAMBP | Affinity Capture-Western, Co-crystal Structure, Reconstituted Complex, Two-hybrid, two hybrid, two hybrid array, two hybrid pooling approach | physical, physical association | 16189514, 16730941, 19060904, 20159979, 27725184, (Europe PMC) | 0.71 | BioGRID, IntAct |
| TGOLN2 | proximity-dependent biotin identification | association | 29568061, (Europe PMC) | 0.35 | IntAct |
| TIMM8A | Two-hybrid, two hybrid array, two hybrid bait and prey pooling approach, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, 25910212, (Europe PMC) | 0.79 | BioGRID, IntAct |
| TMEM17 | Proximity Label-MS, proximity-dependent biotin identification | association, physical | 26638075, (Europe PMC) | 0.35 | BioGRID, IntAct |
| TMEM216 | Proximity Label-MS, proximity-dependent biotin identification | association, physical | 26638075, (Europe PMC) | 0.35 | BioGRID, IntAct |
| TP53 | Synthetic Growth Defect | genetic | 23284306, (Europe PMC) | NA | BioGRID |
| TP53BP2 | Co-fractionation | physical | 26344197, (Europe PMC) | NA | BioGRID |
| TPTE | Proximity Label-MS | physical | 27880917, (Europe PMC) | NA | BioGRID |
| TSG101 | Co-fractionation | physical | 26344197, (Europe PMC) | NA | BioGRID |
| TYK2 | Affinity Capture-Western | physical | 10899310, (Europe PMC) | NA | BioGRID |
| UBC | Co-crystal Structure, Reconstituted Complex, surface plasmon resonance | direct interaction, physical | 12750381, 20504764, 21121635, 22841719, 26601948, 27725184, 28190767, (Europe PMC) | 0.44 | BioGRID, IntAct, MINT |
| UBE2D1 | Biochemical Activity | physical | 17588522, (Europe PMC) | NA | BioGRID |
| UBE2D2 | Biochemical Activity | physical | 17588522, (Europe PMC) | NA | BioGRID |
| UBE2D3 | Biochemical Activity | physical | 17588522, (Europe PMC) | NA | BioGRID |
| UBE2H | Biochemical Activity | physical | 17588522, (Europe PMC) | NA | BioGRID |
| UBQLN1 | Affinity Capture-Western, two hybrid array, two hybrid prey pooling approach | physical, physical association | 16159959, (Europe PMC) | 0.49 | BioGRID, IntAct |
| UBQLN4 | Two-hybrid, two hybrid array | physical, physical association | 16713569, (Europe PMC) | 0.37 | BioGRID, IntAct |
| USP8 | Affinity Capture-Western, PCA, Protein-peptide, Reconstituted Complex | physical | 10982817, 13129930, 20736164, (Europe PMC) | NA | BioGRID |
| VCP | Co-fractionation | physical | 22863883, (Europe PMC) | NA | BioGRID |
| VPS37C | Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid | physical, physical association | 25416956, (Europe PMC) | 0.70 | BioGRID, IntAct |
| XPO1 | Affinity Capture-MS | physical | 26673895, (Europe PMC) | NA | BioGRID |
| XRN1 | Two-hybrid, two hybrid | physical, physical association | 15231747, (Europe PMC) | 0.37 | BioGRID, IntAct |
| ZFYVE9 | Affinity Capture-Western | physical | 23222715, (Europe PMC) | NA | BioGRID |
| Phosphorylating Kinases | Phosphoresidues | Detection Method | PubMed IDs | Source |
| EGFR | Y192_HTETKSLyPSSEIQL, | in vitro, in vivo | 10993906, 11687594, 17389395, 19901323,(Europe PMC) | HPRD, |
| FYN | Y291_KSEPEPVyIDEDKMD, Y371_LVNEAPVySVYSKLH, Y374_EAPVYSVySKLHPPA, | NA | NA | PhosphoSitePlus, |
| Unknown | S372_VNEAPVYsVYSKLHP, Y191_QHTETKSyYPSSEIQ, Y192_HTETKSLyPSSEIQL, Y290_KKSEPEPyYIDEDKM, Y291_KSEPEPVyIDEDKMD, Y371_LVNEAPVySVYSKLH, Y374_EAPVYSVySKLHPPA, | HTP, LTP, in vitro, in vivo | 10993906, 11687594, 15144186, 15592455, 17016520, 17081983, 17389395, 19901323,(Europe PMC) | HPRD, PhosphoELM, |