Top MASTL
Localization (UniProt annotation) Cytoplasm, cytoskeleton, microtubuleorganizing center, centrosome Nucleus Cleavage furrow Note=During interphase is mainlynuclear, upon nuclear envelope breakdown localizes at thecytoplasm and during mitosis at the centrosomes Upon mitotic exitmoves to the cleavage furrow Function (UniProt annotation) Serine/threonine kinase that plays a key role in M phaseby acting as a regulator of mitosis entry and maintenance Acts bypromoting the inactivation of protein phosphatase 2A (PP2A) duringM phase: does not directly inhibit PP2A but acts by mediatingphosphorylation and subsequent activation of ARPP19 and ENSA at'Ser-62' and 'Ser-67', respectively ARPP19 and ENSA arephosphatase inhibitors that specifically inhibit the PPP2R2D(PR55-delta) subunit of PP2A Inactivation of PP2A during M phaseis essential to keep cyclin-B1-CDK1 activity high Following DNAdamage, it is also involved in checkpoint recovery by beinginhibited Phosphorylates histone protein in vitro; however suchactivity is unsure in vivo May be involved in megakaryocytedifferentiation Catalytic Activity (UniProt annotation) ATP + a protein = ADP + a phosphoprotein Protein Sequence MDPTAGSKKEPGGGAATEEGVNRIAVPKPPSIEEFSIVKPISRGAFGKVYLGQKGGKLYAVKVVKKADMINKNMTHQVQA
ERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPD
NMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMAKPRQDYSRTPGQVLSLISSLGFNTPIAEKNQDPANILSACLS
ETSQLSQGLVCPMSVDQKDTTPYSSKLLKSCLETVASNPGMPVKCLTSNLLQSRKRLATSSASSQSHTFISSVESECHSS
PKWEKDCQESDEALGPTMMSWNAVEKLCAKSANAIETKGFNKKDLELALSPIHNSSALPTTGRSCVNLAKKCFSGEVSWE
AVELDVNNINMDTDTSQLGFHQSNQWAVDSGGISEEHLGKRSLKRNFELVDSSPCKKIIQNKKTCVEYKHNEMTNCYTNQ
NTGLTVEVQDLKLSVHKSQQNDCANKENIVNSFTDKQQTPEKLPIPMIAKNLMCELDEDCEKNSKRDYLSSSFLCSDDDR
ASKNISMNSDSSFPGISIMESPLESQPLDSDRSIKESSFEESNIEDPLIVTPDCQEKTSPKGVENPAVQESNQKMLGPPL
EVLKTLASKRNAVAFRSFNSHINASNNSEPSRMNMTSLDAMDISCAYSGSYPMAITPTQKRRSCMPHQQTPNQIKSGTPY
RTPKSVRRGVAPVDDGRILGTPDYLAPELLLGRAHGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEG
EEKLSDNAQSAVEILLTIDDTKRAGMKELKRHPLFSDVDWENLQHQTMPFIPQPDDETDTSYFEARNTAQHLTVSGFSL
ELM Motif Motif Instance Description Motif Regex NA NA NA NA
MASTL is dephosphorylated by following phosphatases:
Pathway ID Pathway Name Pathway Description (KEGG) R-HSA-2465910 MASTL Facilitates Mitotic Progression. The activity of MASTL, also known as the Greatwall kinase (GWL), is necessary for the entry and progression of mitosis. MASTL is activated by phosphorylation of several key residues during mitotic entry. Phosphorylation on the serine residue S875 (S883 in Xenopus), likely through autophosphorylation (Blake-Hodek et al. 2012) appears to be critical (Vigneron et al. 2011). Several other sites, including putative CDK1 targets T194, T207 and T741, contribute to the full activation of MASTL (Yu et al. 2006, Blake-Hodek et al. 2012). Other kinases, such as PLK1 (Vigneron et al. 2011) and other MASTL phosphorylation sites may also be functionally important (Yu et al. 2006, Blake-Hodek et al. 2012).Activated MASTL phosphorylates ARPP19 and ENSA on serines S62 and S67, respectively, enabling them to bind to and inhibit the phosphatase activity of PP2A complexed with the regulatory subunit PPP2R2D (B55-delta). Inhibition of PP2A-PPP2R2D activity by ARPP19 or ENSA prevents dephosphorylation of CDK1 targets, hence allowing entry and maintenance of mitosis (Mochida et al. 2010, Gharbi-Ayachi et al. 2010, Burgess et al. 2010)
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source C16orf70 Affinity Capture-MS physical 26186194 , 28514442 , (Europe PMC )NA BioGRID CDK18 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct CEP85 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct DERL2 Affinity Capture-MS physical 26496610 , (Europe PMC )NA BioGRID DYNLT1 Proximity Label-MS physical 26638075 , (Europe PMC )NA BioGRID EPB41L5 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct FASTKD5 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct FBXW7 Synthetic Lethality genetic 26354767 , (Europe PMC )NA BioGRID ITPRID2 Affinity Capture-MS physical 26496610 , (Europe PMC )NA BioGRID LMNA Proximity Label-MS physical 22412018 , (Europe PMC )NA BioGRID MED26 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct MRPL2 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct PLEKHA8 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct PRKCI Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct RPS6KA1 Affinity Capture-MS, tandem affinity purification association, physical 23455922 , (Europe PMC )0.35 BioGRID, IntAct SLC1A5 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct TAP2 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct TELO2 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct TMEM263 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct UBN1 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source CDK18 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct CDK8 proximity-dependent biotin identification association 29568061 , (Europe PMC )0.35 IntAct CEP85 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct DERL2 anti tag coimmunoprecipitation association 26496610 , (Europe PMC )0.35 IntAct EGLN3 anti tag coimmunoprecipitation association 26972000 , (Europe PMC )0.35 IntAct EPB41L5 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct FASTKD5 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct MED26 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct MRPL2 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct PLEKHA8 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct PRKCI Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct RPS6KA1 Affinity Capture-MS, tandem affinity purification association, physical 23455922 , (Europe PMC )0.35 BioGRID, IntAct SLC1A5 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct SSFA2 anti tag coimmunoprecipitation association 26496610 , (Europe PMC )0.35 IntAct TAP2 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct TCTE1 proximity-dependent biotin identification association 26638075 , (Europe PMC )0.35 IntAct TELO2 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct TMEM263 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct UBN1 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source C16orf70 Affinity Capture-MS physical 26186194 , 28514442 , (Europe PMC )NA BioGRID CDK18 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct CDK8 proximity-dependent biotin identification association 29568061 , (Europe PMC )0.35 IntAct CEP85 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct DERL2 Affinity Capture-MS physical 26496610 , (Europe PMC )NA BioGRID DERL2 anti tag coimmunoprecipitation association 26496610 , (Europe PMC )0.35 IntAct DYNLT1 Proximity Label-MS physical 26638075 , (Europe PMC )NA BioGRID EGLN3 anti tag coimmunoprecipitation association 26972000 , (Europe PMC )0.35 IntAct EPB41L5 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct FASTKD5 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct FBXW7 Synthetic Lethality genetic 26354767 , (Europe PMC )NA BioGRID ITPRID2 Affinity Capture-MS physical 26496610 , (Europe PMC )NA BioGRID LMNA Proximity Label-MS physical 22412018 , (Europe PMC )NA BioGRID MED26 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct MRPL2 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct PLEKHA8 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct PRKCI Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct RPS6KA1 Affinity Capture-MS, tandem affinity purification association, physical 23455922 , (Europe PMC )0.35 BioGRID, IntAct SLC1A5 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct SSFA2 anti tag coimmunoprecipitation association 26496610 , (Europe PMC )0.35 IntAct TAP2 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct TCTE1 proximity-dependent biotin identification association 26638075 , (Europe PMC )0.35 IntAct TELO2 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct TMEM263 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct UBN1 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct
Phosphorylating Kinases Phosphoresidues Detection Method PubMed IDs Source
Visualize a network of protein kinases and phosphatases phosphorylating and dephosphorylating this protein respectively
Unknown S213_RTPGQVLsLISSLGF , S217_QVLSLISsLGFNTPI , S293_LTSNLLQsRKRLATS , S370_KDLELALsPIHNSSA , S375_ALSPIHNsSALPTTG , S452_RNFELVDsSPCKKII , S453_NFELVDSsPCKKIIQ , S512_NKENIVNsFTDKQQT , S552_KRDYLSSsFLCSDDD , S556_LSSSFLCsDDDRASK , S631_ENPAVQEsNQKMLGP , S657_RNAVAFRsFNSHINA , S665_FNSHINAsNNSEPSR , S668_HINASNNsEPSRMNM , S724_GTPYRTPsSVRRGVA , S725_TPYRTPKsVRRGVAP , S874_NTAQHLTsSGFSL , S875_TAQHLTVsGFSL , S877_QHLTVSGsSL , S878_HLTVSGFsL , T194_NMMDILTtPSMAKPR , T207_PRQDYSRtPGQVLSL , T222_ISSLGFNtPIAEKNQ , T514_ENIVNSFtDKQQTPE , T519_SFTDKQQtPEKLPIP , T618_TPDCQEKtSPKGVEN , T721_IKSGTPYtTPKSVRR , T722_KSGTPYRtPKSVRRG , T740_VDDGRILtTPDYLAP , T741_DDGRILGtPDYLAPE , Y548_EKNSKRDyLSSSFLC , Y719_NQIKSGTyYRTPKSV , Y720_QIKSGTPyRTPKSVR , HTP, in vivo 16964243 , 17322306 , 18220336 , 18669648 , 18691976 , 18767875 , 19007248 , 19651622 , 19664994 , 19664995 , 20068231 ,(Europe PMC )HPRD, PhosphoELM ,
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