DEPOD - human DEPhOsphorylation Database

Basic Information
Phosphatases
Pathway
Interacting Proteins
Phosphorylating Kinases

Gene Name	ARHGAP12 (QuickGO)	Interactive visualization of ARHGAP12 structures (A quick tutorial to explore the interctive visulaization) Representative structure: NA
Synonyms	ARHGAP12
Protein Name	ARHGAP12
Alternative Name(s)	Rho GTPase-activating protein 12;Rho-type GTPase-activating protein 12;
Protein Family	noSimilarity_annotations
EntrezGene ID	94134 (Comparitive Toxicogenomics)
UniProt AC (Human)	Q8IWW6 (protein sequence)
Enzyme Class	N/A
Molecular Weight	96254 Dalton
Protein Length	846 amino acids (AA)
Genome Browsers	NCBI \| ENSG00000165322 (Ensembl) \| UCSC
Crosslinking annotations	Query our ID-mapping table
Orthologues	Quest For Orthologues (QFO) \| GeneTree \| eggNOG - ENOG410IQ9D \| eggNOG - ENOG410ZTHP
Phosphorylation Network	Visualize

Domain organization, Expression, Diseases

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Protein Domain	InterPro \| Pfam \| SMART	3D Structure	PDB \| DrugPort \| ModBase \| SwissModel
Polypeptide organization (Pfam)
Gene Expression	Gene Expression Atlas The Human Protein Atlas	Disease	OMIM \| COSMIC \| ClinVar
Protein-protein interactions	STRING \| IntAct \| MINT \| BioGRID
Phosphosites	Phospho.ELM \| PhosphoSitePlus

Localization, Function, Catalytic activity and Sequence

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Localization (UniProt annotation)
N/A
Function (UniProt annotation)
GTPase activator for the Rho-type GTPases by convertingthem to an inactive GDP-bound state
Catalytic Activity (UniProt annotation)
N/A
Protein Sequence
MKMADRSGKIIPGQVYIEVEYDYEYEAKDRKIVIKQGERYILVKKTNDDWWQVKPDENSKAFYVPAQYVKEVTRKALMPP VKQVAGLPNNSTKIMQSLHLQRSTENVNKLPELSSFGKPSSSVQGTGLIRDANQNFGPSYNQGQTVNLSLDLTHNNGKFN NDSHSPKVSSQNRTRSFGHFPGPEFLDVEKTSFSQEQSCDSAGEGSERIHQDSESGDELSSSSTEQIRATTPPNQGRPDS PVYANLQELKISQSALPPLPGSPAIQINGEWETHKDSSGRCYYYNRGTQERTWKPPRWTRDASISKGDFQNPGDQELLSS EENYYSTSYSQSDSQCGSPPRGWSEELDERGHTLYTSDYTNEKWLKHVDDQGRQYYYSADGSRSEWELPKYNASSQQQRE IIKSRSLDRRLQEPIVLTKWRHSTIVLDTNDKESPTASKPCFPENESSPSSPKHQDTASSPKDQEKYGLLNVTKIAENGK KVRKNWLSSWAVLQGSSLLFTKTQGSSTSWFGSNQSKPEFTVDLKGATIEMASKDKSSKKNVFELKTRQGTELLIQSDND TVINDWFKVLSSTINNQAVETDEGIEEEIPDSPGIEKHDKEKEQKDPKKLRSFKVSSIDSSEQKKTKKNLKKFLTRRPTL QAVREKGYIKDQVFGSNLANLCQRENGTVPKFVKLCIEHVEEHGLDIDGIYRVSGNLAVIQKLRFAVNHDEKLDLNDSKW EDIHVITGALKMFFRELPEPLFTFNHFNDFVNAIKQEPRQRVAAVKDLIRQLPKPNQDTMQILFRHLRRVIENGEKNRMT YQSIAIVFGPTLLKPEKETGNIAVHTVYQNQIVELILLELSSIFGR

Motif information from Eukaryotic Linear Motif atlas (ELM)

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ELM Motif	Motif Instance	Description	Motif Regex
NA	NA	NA	NA

Gene Ontology (P: Process; F: Function and C: Component terms)

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GO:0001891	C:phagocytic cup
GO:0002011	P:morphogenesis of an epithelial sheet
GO:0005096	F:GTPase activator activity
GO:0005829	C:cytosol
GO:0006911	P:phagocytosis
GO:0007015	engulfment
GO:0007165	P:actin filament organization
GO:0051056	P:signal transduction
GO:0051058	P:regulation of small GTPase mediated signal transduction

ARHGAP12 is dephosphorylated by following phosphatases:

Phosphatase Gene Name	Phosphatase UniProt_AC	DephosphoSite and sequence (+/-5) in ARHGAP12 (Q8IWW6)	Bioassay Type	PubMed ID	View in Europe PMC	Reliability of the interaction
PTPN1	P18031	NA	In vitro	18515860,	Europe PMC

KEGG Pathway
Reactome Pathway

Sorry, no KEGG pathway information here!
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Pathway ID

Pathway Name

Pathway Description (KEGG)

R-HSA-194840

Rho GTPase cycle.

The cycling of Rho GTPases is tightly controlled by three classes of protein. These are (1) guanine nucleotide dissociation inhibitors or GDIs, which maintain Rho proteins in an inactive state in the cytoplasm, (2) guanine nucleotide exchange factors or GEFs, which destabilize the interaction between Rho proteins and their bound nucleotide, the net result of which is the exchange of bound GDP for the more abundant GTP, and (3) GTPase Activating Proteins or GAPs, which stimulate the low intrinsic GTP hydrolysis activity of Rho family members, thus promoting their inactivation. GDIs, GEFs, and GAPs are themselves subject to tight regulation, and the overall level of Rho activity reflects the balance of their activities.

In their active GTP-bound state, Rho family members have the ability to interact with a large variety of so-called effector proteins. By changing the subcellular localization of effectors, by altering their enzymatic properties, or by directing the formation of specific effector complexes, members of the Rho family mediate their various effects.

This Rho GTPase cycle is diagrammed in the figure below. External or internal cues promote the release of Rho GTPases from the inhibitory complex (1) which allows them to associate with the plasma membrane (2) where they are activated by GEFs (3) and can signal to effector proteins. Then, GAPs inactivate the GTPases by accelerating the intrinsic GTPase activity, leading to the GDP bound form (4). Once again, the GDI molecules stabilize the inactive GDP bound form in the cytoplasm, waiting for further instructions (5). (Figure and text from Tcherkezian and Lamarche Vane, 2007)

BioGRID
IntAct
MINT
ALL

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Interacting partner	Detection method	Interaction type	PubMed IDs	Interaction Score	Source
DMBT1	Affinity Capture-MS	physical	28514442, (Europe PMC)	NA	BioGRID
GRB2	Affinity Capture-MS	physical	21706016, (Europe PMC)	NA	BioGRID
IGHA1	Affinity Capture-MS	physical	28514442, (Europe PMC)	NA	BioGRID
MAPK11	Affinity Capture-MS, tandem affinity purification	association, physical	23602568, (Europe PMC)	0.35	BioGRID, IntAct
MUC5B	Affinity Capture-MS	physical	28514442, (Europe PMC)	NA	BioGRID
SRPK1	Biochemical Activity	physical	23602568, (Europe PMC)	NA	BioGRID
SRPK2	Biochemical Activity	physical	23602568, (Europe PMC)	NA	BioGRID
TRIP10	Two-hybrid, two hybrid array, two hybrid prey pooling approach	physical, physical association	25416956, (Europe PMC)	0.49	BioGRID, IntAct
XPO1	Affinity Capture-MS	physical	26673895, (Europe PMC)	NA	BioGRID

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Interacting partner	Detection method	Interaction type	PubMed IDs	Interaction Score	Source
SRPK1	protein kinase assay	phosphorylation reaction	23602568, (Europe PMC)	0.44	IntAct
SRPK2	protein kinase assay	phosphorylation reaction	23602568, (Europe PMC)	0.44	IntAct
TRIP10	Two-hybrid, two hybrid array, two hybrid prey pooling approach	physical, physical association	25416956, (Europe PMC)	0.49	BioGRID, IntAct

Sorry, no MINT interacting protein information here!
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Interacting partner	Detection method	Interaction type	PubMed IDs	Interaction Score	Source
DMBT1	Affinity Capture-MS	physical	28514442, (Europe PMC)	NA	BioGRID
GRB2	Affinity Capture-MS	physical	21706016, (Europe PMC)	NA	BioGRID
IGHA1	Affinity Capture-MS	physical	28514442, (Europe PMC)	NA	BioGRID
MAPK11	Affinity Capture-MS, tandem affinity purification	association, physical	23602568, (Europe PMC)	0.35	BioGRID, IntAct
MUC5B	Affinity Capture-MS	physical	28514442, (Europe PMC)	NA	BioGRID
SRPK1	Biochemical Activity	physical	23602568, (Europe PMC)	NA	BioGRID
SRPK1	protein kinase assay	phosphorylation reaction	23602568, (Europe PMC)	0.44	IntAct
SRPK2	Biochemical Activity	physical	23602568, (Europe PMC)	NA	BioGRID
SRPK2	protein kinase assay	phosphorylation reaction	23602568, (Europe PMC)	0.44	IntAct
TRIP10	Two-hybrid, two hybrid array, two hybrid prey pooling approach	physical, physical association	25416956, (Europe PMC)	0.49	BioGRID, IntAct
XPO1	Affinity Capture-MS	physical	26673895, (Europe PMC)	NA	BioGRID

Visualize a network of protein kinases and phosphatases phosphorylating and dephosphorylating this protein respectively

Phosphorylating Kinases	Phosphoresidues	Detection Method	PubMed IDs	Source
Unknown	S103_QSLHLQRsTENVNKL, S165_KFNNDSHsPKVSSQN, S176_SSQNRTRsFGHFPGP, S201_SQEQSCDsAGEGSER, S213_SERIHQDsESGDELS, S215_RIHQDSEsGDELSSS, S223_GDELSSSsTEQIRAT, S240_PNQGRPDsPVYANLQ, S262_ALPPLPGsPAIQING, S395_LPKYNASsQQQREII, S404_QQREIIKsRSLDRRL, S592_IEEEIPDsPGIEKHD, S91_VAGLPNNsTKIMQSL, T104_SLHLQRStENVNKLP, T230_STEQIRAtTPPNQGR, T231_TEQIRATtPPNQGRP, Y243_GRPDSPVyANLQELK,	HTP, in vitro, in vivo	15144186, 15345747, 17081983, 18452278, 18669648, 19413330, 19415658, 19651622, 20068231,(Europe PMC)	HPRD, PhosphoELM,

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ARHGAP12