Top PTPN3
Download FASTA sequences of PTPN3-substrates in Humans
Pathway ID Pathway Name Pathway Description (KEGG) R-HSA-182971 EGFR downregulation. Regulation of receptor tyrosine kinase (RTK) activity is implicated in the control of almost all cellular functions. One of the best understood RTKs is epidermal growth factor receptor (EGFR). Growth factors can bind to EGFR and activate it to initiate signalling cascades within the cell. EGFRs can also be recruited to clathrin-coated pits which can be internalised into endocytic vesicles. From here, EGFRs can either be recycled back to the plasma membrane or directed to lysosomes for destruction.This provides a mechanism by which EGFR signalling is negatively regulated and controls the strength and duration of EGFR-induced signals. It also prevents EGFR hyperactivation as commonly seen in tumorigenesis.The proto-oncogene Cbl can negatively regulate EGFR signalling. The Cbl family of RING-type ubiquitin ligases are able to poly-ubiquitinate EGFR, an essential step in EGFR degradation. All Cbl proteins have a unique domain that recognises phosphorylated tyrosine residues on activated EGFRs. They also direct the ubiquitination and degradation of activated EGFRs by recruiting ubiquitin-conjugation enzymes. Cbl proteins function by specifically targeting activated EGFRs and mediating their down-regulation, thus providing a means by which signaling processes can be negatively regulated.Cbl also promotes receptor internalization via it's interaction with an adaptor protein, CIN85 (Cbl-interacting protein of 85kDa). CIN85 binds to Cbl via it's SH3 domain and is enhanced by the EGFR-induced tyrosine phosphorylation of Cbl. The proline-rich region of CIN85 interacts with endophilins which are regulatory components of clathrin-coated vesicles (CCVs). Endophilins bind to membranes and induce membrane curvature, in conjunction with other proteins involved in CCV formation. The rapid recruitment of endophilin to the activated receptor complex by CIN85 is the mechanism which controls receptor internalization
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source AKR1C1 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct AKT1 Two-hybrid, two hybrid array physical, physical association 25640309 , (Europe PMC )0.37 BioGRID, IntAct, MINT CACYBP Affinity Capture-MS physical 27432908 , (Europe PMC )NA BioGRID DIP2A Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , (Europe PMC )0.56 BioGRID, IntAct GHR Far Western, Reconstituted Complex, array technology, far western blotting, phosphatase assay dephosphorylation reaction, direct interaction, physical, physical association 12907755 , (Europe PMC )0.65 BioGRID, IntAct HSPB1 Two-hybrid, reverse ras recruitment system physical, physical association 25277244 , (Europe PMC )0.37 BioGRID, IntAct IGFBP1 Affinity Capture-MS physical 28514442 , (Europe PMC )NA BioGRID KCTD3 Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct LMO1 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct NXT2 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , (Europe PMC )0.56 BioGRID, IntAct PTPN3 Affinity Capture-MS physical 27432908 , (Europe PMC )NA BioGRID SFN Affinity Capture-MS, tandem affinity purification physical, physical association 15778465 , (Europe PMC )0.40 BioGRID, IntAct, MINT SHKBP1 Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct SLC1A5 Affinity Capture-MS physical 27432908 , 27880917 , (Europe PMC )NA BioGRID SRRM2 Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct TRIM25 Affinity Capture-RNA physical 29117863 , (Europe PMC )NA BioGRID UBE3A Affinity Capture-MS physical 17166906 , (Europe PMC )NA BioGRID VANGL1 Affinity Capture-MS, anti tag coimmunoprecipitation, proximity-dependent biotin identification, pull down association, physical, physical association 27432908 , 27880917 , 28330616 , (Europe PMC )0.56 BioGRID, IntAct VDR Affinity Capture-Western, anti tag coimmunoprecipitation, pull down physical, physical association 21119599 , (Europe PMC )0.52 BioGRID, IntAct WDR6 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct YWHAB Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex, Two-hybrid, anti bait coimmunoprecipitation, proximity-dependent biotin identification, pull down association, physical, physical association 17353931 , 27432908 , 27880917 , 28330616 , 9341175 , (Europe PMC )0.71 BioGRID, IntAct, MINT YWHAE Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct YWHAG Affinity Capture-MS, Two-hybrid, anti bait coimmunoprecipitation, proximity-dependent biotin identification, pull down, two hybrid pooling approach association, physical, physical association 17353931 , 20936779 , 27432908 , 27880917 , 28330616 , (Europe PMC )0.72 BioGRID, IntAct YWHAH Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct YWHAQ Affinity Capture-MS, Reconstituted Complex, proximity-dependent biotin identification, pull down association, physical 15161933 , 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct YWHAZ Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical, physical association 15161933 , 27432908 , 27880917 , 28330616 , (Europe PMC )0.62 BioGRID, IntAct, MINT ZCCHC10 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , (Europe PMC )0.56 BioGRID, IntAct
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source AKR1C1 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct AKT1 Two-hybrid, two hybrid array physical, physical association 25640309 , (Europe PMC )0.37 BioGRID, IntAct, MINT DIP2A Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , (Europe PMC )0.56 BioGRID, IntAct EIF3A proximity-dependent biotin identification, pull down association 28330616 , (Europe PMC )0.46 IntAct EIF3B proximity-dependent biotin identification, pull down association 28330616 , (Europe PMC )0.46 IntAct EIF5A pull down association 28330616 , (Europe PMC )0.35 IntAct EPS15 anti tag coimmunoprecipitation, fluorescence polarization spectroscopy, phosphatase assay, x-ray crystallography dephosphorylation reaction, direct interaction, physical association 25728925 , (Europe PMC )0.65 IntAct ERBB2 phosphatase assay dephosphorylation reaction 19167335 , (Europe PMC )0.44 IntAct EXPH5 pull down association 28330616 , (Europe PMC )0.35 IntAct GHR Far Western, Reconstituted Complex, array technology, far western blotting, phosphatase assay dephosphorylation reaction, direct interaction, physical, physical association 12907755 , (Europe PMC )0.65 BioGRID, IntAct HEATR5B proximity-dependent biotin identification, pull down association 28330616 , (Europe PMC )0.46 IntAct HSPB1 Two-hybrid, reverse ras recruitment system physical, physical association 25277244 , (Europe PMC )0.37 BioGRID, IntAct ILF2 proximity-dependent biotin identification, pull down association 28330616 , (Europe PMC )0.46 IntAct ILF3 proximity-dependent biotin identification, pull down association 28330616 , (Europe PMC )0.46 IntAct INSR phosphatase assay dephosphorylation reaction 25728925 , (Europe PMC )0.44 IntAct KCTD3 Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct LMO1 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct MAPK12 phosphatase assay dephosphorylation reaction 25728925 , (Europe PMC )0.44 IntAct MCC anti tag coimmunoprecipitation, pull down association, physical association 28330616 , (Europe PMC )0.50 IntAct MET phosphatase assay dephosphorylation reaction 19167335 , (Europe PMC )0.44 IntAct NXT2 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , (Europe PMC )0.56 BioGRID, IntAct PRKD2 two hybrid physical association 15231748 , (Europe PMC )0.37 IntAct, MINT SCML1 proximity-dependent biotin identification, pull down association 28330616 , (Europe PMC )0.46 IntAct SCN5A pull down, two hybrid direct interaction, physical association 16930557 , (Europe PMC )0.53 IntAct, MINT SDHA anti tag coimmunoprecipitation, pull down association, physical association 28330616 , (Europe PMC )0.50 IntAct SFN Affinity Capture-MS, tandem affinity purification physical, physical association 15778465 , (Europe PMC )0.40 BioGRID, IntAct, MINT SHKBP1 Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct SRRM2 Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct VANGL1 Affinity Capture-MS, anti tag coimmunoprecipitation, proximity-dependent biotin identification, pull down association, physical, physical association 27432908 , 27880917 , 28330616 , (Europe PMC )0.56 BioGRID, IntAct VANGL2 anti tag coimmunoprecipitation, pull down association, physical association 28330616 , (Europe PMC )0.50 IntAct VCP anti tag coimmunoprecipitation, pull down physical association 10364224 , (Europe PMC )0.52 IntAct VDR Affinity Capture-Western, anti tag coimmunoprecipitation, pull down physical, physical association 21119599 , (Europe PMC )0.52 BioGRID, IntAct WDR6 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct YWHAB Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex, Two-hybrid, anti bait coimmunoprecipitation, proximity-dependent biotin identification, pull down association, physical, physical association 17353931 , 27432908 , 27880917 , 28330616 , 9341175 , (Europe PMC )0.71 BioGRID, IntAct, MINT YWHAE Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct YWHAG Affinity Capture-MS, Two-hybrid, anti bait coimmunoprecipitation, proximity-dependent biotin identification, pull down, two hybrid pooling approach association, physical, physical association 17353931 , 20936779 , 27432908 , 27880917 , 28330616 , (Europe PMC )0.72 BioGRID, IntAct YWHAH Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct YWHAQ Affinity Capture-MS, Reconstituted Complex, proximity-dependent biotin identification, pull down association, physical 15161933 , 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct YWHAZ Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical, physical association 15161933 , 27432908 , 27880917 , 28330616 , (Europe PMC )0.62 BioGRID, IntAct, MINT ZCCHC10 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , (Europe PMC )0.56 BioGRID, IntAct ZNF512B two hybrid physical association 15231748 , (Europe PMC )0.37 IntAct, MINT
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source AKT1 Two-hybrid, two hybrid array physical, physical association 25640309 , (Europe PMC )0.37 BioGRID, IntAct, MINT PRKD2 two hybrid physical association 15231748 , (Europe PMC )0.37 IntAct, MINT SCN5A pull down, two hybrid direct interaction, physical association 16930557 , (Europe PMC )0.53 IntAct, MINT SFN Affinity Capture-MS, tandem affinity purification physical, physical association 15778465 , (Europe PMC )0.40 BioGRID, IntAct, MINT YWHAB Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex, Two-hybrid, anti bait coimmunoprecipitation, proximity-dependent biotin identification, pull down association, physical, physical association 17353931 , 27432908 , 27880917 , 28330616 , 9341175 , (Europe PMC )0.71 BioGRID, IntAct, MINT YWHAZ Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical, physical association 15161933 , 27432908 , 27880917 , 28330616 , (Europe PMC )0.62 BioGRID, IntAct, MINT ZNF512B two hybrid physical association 15231748 , (Europe PMC )0.37 IntAct, MINT
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source ADAM17 anti tag coimmunoprecipitation, two hybrid physical association 12207026 , (Europe PMC )0.37, 0.40 IntAct, MINT AKR1C1 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct AKT1 Two-hybrid, two hybrid array physical, physical association 25640309 , (Europe PMC )0.37 BioGRID, IntAct, MINT CACYBP Affinity Capture-MS physical 27432908 , (Europe PMC )NA BioGRID DIP2A Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , (Europe PMC )0.56 BioGRID, IntAct EIF3A proximity-dependent biotin identification, pull down association 28330616 , (Europe PMC )0.46 IntAct EIF3B proximity-dependent biotin identification, pull down association 28330616 , (Europe PMC )0.46 IntAct EIF5A pull down association 28330616 , (Europe PMC )0.35 IntAct EPS15 anti tag coimmunoprecipitation, fluorescence polarization spectroscopy, phosphatase assay, x-ray crystallography dephosphorylation reaction, direct interaction, physical association 25728925 , (Europe PMC )0.65 IntAct ERBB2 phosphatase assay dephosphorylation reaction 19167335 , (Europe PMC )0.44 IntAct EXPH5 pull down association 28330616 , (Europe PMC )0.35 IntAct GHR Far Western, Reconstituted Complex, array technology, far western blotting, phosphatase assay dephosphorylation reaction, direct interaction, physical, physical association 12907755 , (Europe PMC )0.65 BioGRID, IntAct HEATR5B proximity-dependent biotin identification, pull down association 28330616 , (Europe PMC )0.46 IntAct HSPB1 Two-hybrid, reverse ras recruitment system physical, physical association 25277244 , (Europe PMC )0.37 BioGRID, IntAct IGFBP1 Affinity Capture-MS physical 28514442 , (Europe PMC )NA BioGRID ILF2 proximity-dependent biotin identification, pull down association 28330616 , (Europe PMC )0.46 IntAct ILF3 proximity-dependent biotin identification, pull down association 28330616 , (Europe PMC )0.46 IntAct INSR phosphatase assay dephosphorylation reaction 25728925 , (Europe PMC )0.44 IntAct KCTD3 Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct LMO1 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct MAPK12 phosphatase assay dephosphorylation reaction 25728925 , (Europe PMC )0.44 IntAct MCC anti tag coimmunoprecipitation, pull down association, physical association 28330616 , (Europe PMC )0.50 IntAct MET phosphatase assay dephosphorylation reaction 19167335 , (Europe PMC )0.44 IntAct NXT2 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , (Europe PMC )0.56 BioGRID, IntAct PRKD2 two hybrid physical association 15231748 , (Europe PMC )0.37 IntAct, MINT PTPN3 Affinity Capture-MS physical 27432908 , (Europe PMC )NA BioGRID SCML1 proximity-dependent biotin identification, pull down association 28330616 , (Europe PMC )0.46 IntAct SCN5A pull down, two hybrid direct interaction, physical association 16930557 , (Europe PMC )0.53 IntAct, MINT SDHA anti tag coimmunoprecipitation, pull down association, physical association 28330616 , (Europe PMC )0.50 IntAct SFN Affinity Capture-MS, tandem affinity purification physical, physical association 15778465 , (Europe PMC )0.40 BioGRID, IntAct, MINT SHKBP1 Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct SLC1A5 Affinity Capture-MS physical 27432908 , 27880917 , (Europe PMC )NA BioGRID SRRM2 Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct TRIM25 Affinity Capture-RNA physical 29117863 , (Europe PMC )NA BioGRID UBE3A Affinity Capture-MS physical 17166906 , (Europe PMC )NA BioGRID VANGL1 Affinity Capture-MS, anti tag coimmunoprecipitation, proximity-dependent biotin identification, pull down association, physical, physical association 27432908 , 27880917 , 28330616 , (Europe PMC )0.56 BioGRID, IntAct VANGL2 anti tag coimmunoprecipitation, pull down association, physical association 28330616 , (Europe PMC )0.50 IntAct VCP anti tag coimmunoprecipitation, pull down physical association 10364224 , (Europe PMC )0.52 IntAct VDR Affinity Capture-Western, anti tag coimmunoprecipitation, pull down physical, physical association 21119599 , (Europe PMC )0.52 BioGRID, IntAct WDR6 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct YWHAB Affinity Capture-MS, Affinity Capture-Western, Reconstituted Complex, Two-hybrid, anti bait coimmunoprecipitation, proximity-dependent biotin identification, pull down association, physical, physical association 17353931 , 27432908 , 27880917 , 28330616 , 9341175 , (Europe PMC )0.71 BioGRID, IntAct, MINT YWHAE Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct YWHAG Affinity Capture-MS, Two-hybrid, anti bait coimmunoprecipitation, proximity-dependent biotin identification, pull down, two hybrid pooling approach association, physical, physical association 17353931 , 20936779 , 27432908 , 27880917 , 28330616 , (Europe PMC )0.72 BioGRID, IntAct YWHAH Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct YWHAQ Affinity Capture-MS, Reconstituted Complex, proximity-dependent biotin identification, pull down association, physical 15161933 , 27432908 , 27880917 , 28330616 , (Europe PMC )0.46 BioGRID, IntAct YWHAZ Affinity Capture-MS, proximity-dependent biotin identification, pull down association, physical, physical association 15161933 , 27432908 , 27880917 , 28330616 , (Europe PMC )0.62 BioGRID, IntAct, MINT ZCCHC10 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , (Europe PMC )0.56 BioGRID, IntAct ZNF512B two hybrid physical association 15231748 , (Europe PMC )0.37 IntAct, MINT
Phosphorylating Kinases Phosphoresidues Detection Method PubMed IDs Source
Visualize a network of protein kinases and phosphatases phosphorylating and dephosphorylating this protein respectively
MAP3K7 S359_PAMRRSLsVEHLETK , NA NA PhosphoSitePlus , MAPK12 S459_QKSSSSVsPSSNAPG , NA NA PhosphoSitePlus , Unknown S357_WNPAMRRsLSVEHLE , S359_PAMRRSLsVEHLETK , S367_VEHLETKsLPSRSPP , S370_LETKSLPsRSPPITP , S372_TKSLPSRsPPITPNW , S381_PITPNWRsPRLRHEI , S394_EIRKPRHsSADNLAN , S395_IRKPRHSsADNLANE , S835_VRSLRVDsEPVLVHC , T376_PSRSPPItPNWRSPR , Y324_EKNVLSQyWTMGSRN , HTP, LTP, in vivo 18669648 , 19901323 , 9341175 ,(Europe PMC )HPRD, PhosphoELM ,
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