Top PHLPP1
Download FASTA sequences of PHLPP1-substrates in HumansSubstrate Gene Name Substrate UniProt_AC DephosphoSite and sequence (+/-5) Bioassay Type PubMed ID View in Europe PMC Reliability of the interaction AKT3 Q9Y243 Ser-472 _HFPQFsYSASG , In vitro and in vivo 17386267 , 19261608 , Europe PMC AKT2 P31751 Ser-474 _HFPQFsYSASI , In vitro and in vivo 17386267 , 19261608 , Europe PMC PRKCA P17252 Ser-657_DFEGFsYVNPQ , In vitro and in vivo 18162466 , Europe PMC STK4 Q13043 Thr-387_KRRDEtMQPAK , In vitro and in vivo 20513427 , Europe PMC PRKCB P05771 Ser-660 in Isoform Beta-II _EFEGFsFVNSE , In vitro and in vivo 18162466 , Europe PMC AKT1 P31749 Ser-473_HFPQFsYSASG , In vitro and in vivo 15808505 , Europe PMC RPS6KB1 P23443 Thr-252_GTVTHtFCGTI, , Thr-412_VFLGFtYVAPS , In vitro 21986499 , Europe PMC
Pathway ID Pathway Name Pathway Description (KEGG) hsa04151 PI3K-Akt signaling pathway The phosphatidylinositol 3' -kinase(PI3K)-Akt signaling pathway is activated by many types of cellular stimuli or toxic insults and regulates fundamental cellular functions such as transcription, translation, proliferation, growth, and survival. The binding of growth factors to their receptor tyrosine kinase (RTK) or G protein-coupled receptors (GPCR) stimulates class Ia and Ib PI3K isoforms, respectively. PI3K catalyzes the production of phosphatidylinositol-3,4,5-triphosphate (PIP3) at the cell membrane. PIP3 in turn serves as a second messenger that helps to activate Akt. Once active, Akt can control key cellular processes by phosphorylating substrates involved in apoptosis, protein synthesis, metabolism, and cell cycle.
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source AIP Proximity Label-MS physical 27880917 , (Europe PMC )NA BioGRID AKT1 Affinity Capture-Western, Biochemical Activity physical 15808505 , 20605778 , 27097374 , (Europe PMC )NA BioGRID BAG2 Affinity Capture-MS physical 27880917 , (Europe PMC )NA BioGRID BRAP Reconstituted Complex, Two-hybrid physical 25820252 , (Europe PMC )NA BioGRID BTRC Affinity Capture-Western physical 19797085 , 23440515 , (Europe PMC )NA BioGRID BUD23 Co-fractionation physical 22939629 , (Europe PMC )NA BioGRID COA1 Affinity Capture-MS physical 24145035 , (Europe PMC )NA BioGRID CSNK1A1 Biochemical Activity physical 19797085 , (Europe PMC )NA BioGRID DMWD Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , (Europe PMC )0.40 BioGRID, IntAct EFNB2 Affinity Capture-MS physical 26186194 , 28514442 , (Europe PMC )NA BioGRID ELAVL1 Affinity Capture-RNA physical 19322201 , (Europe PMC )NA BioGRID FANCI Affinity Capture-Western physical 27097374 , (Europe PMC )NA BioGRID FKBP4 Affinity Capture-MS physical 27880917 , (Europe PMC )NA BioGRID FKBP5 Affinity Capture-MS, Affinity Capture-Western, anti tag coimmunoprecipitation physical, physical association 19615732 , 27880917 , 28363942 , (Europe PMC )0.40 BioGRID, IntAct GLMN Proximity Label-MS physical 27880917 , (Europe PMC )NA BioGRID GSK3B Biochemical Activity physical 19797085 , (Europe PMC )NA BioGRID HAVCR2 Affinity Capture-MS physical 28514442 , (Europe PMC )NA BioGRID HDAC1 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct IRS4 Affinity Capture-MS physical 24145035 , (Europe PMC )NA BioGRID MAPK8 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct MARK2 Affinity Capture-MS physical 19615732 , (Europe PMC )NA BioGRID MCMBP Affinity Capture-MS physical 27880917 , (Europe PMC )NA BioGRID NUDC Proximity Label-MS physical 27880917 , (Europe PMC )NA BioGRID NUDCD3 Affinity Capture-MS, Proximity Label-MS physical 27880917 , (Europe PMC )NA BioGRID PHLPP2 Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct PPP2R1A Affinity Capture-MS physical 27880917 , (Europe PMC )NA BioGRID PPP2R2A Affinity Capture-MS physical 27880917 , (Europe PMC )NA BioGRID PPP5C Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , (Europe PMC )0.40 BioGRID, IntAct RBM15 Affinity Capture-MS, Proximity Label-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct RFPL2 Affinity Capture-MS physical 26186194 , 28514442 , (Europe PMC )NA BioGRID RPAP3 Affinity Capture-MS physical 27880917 , (Europe PMC )NA BioGRID SCRIB Affinity Capture-MS, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation physical, physical association 19615732 , 21701506 , 24145035 , (Europe PMC )0.59 BioGRID, IntAct, MINT SKP2 Affinity Capture-MS physical 28514442 , (Europe PMC )NA BioGRID SLC9A3R1 Affinity Capture-Western, Far Western, Reconstituted Complex, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, filter binding, fluorescence microscopy, pull down colocalization, direct interaction, physical, physical association 21804599 , (Europe PMC )0.52, 0.62 BioGRID, IntAct SLC9A3R2 Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct SNX27 Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , (Europe PMC )0.40 BioGRID, IntAct SNX6 Co-fractionation physical 22939629 , (Europe PMC )NA BioGRID STUB1 Affinity Capture-MS physical 24145035 , (Europe PMC )NA BioGRID SUGT1 Affinity Capture-MS, Affinity Capture-Western, Proximity Label-MS physical 19615732 , 23440515 , 24145035 , 27880917 , (Europe PMC )NA BioGRID TRIM25 Affinity Capture-RNA physical 29117863 , (Europe PMC )NA BioGRID USP1 Affinity Capture-MS, Affinity Capture-Western, Proximity Label-MS, Reconstituted Complex, anti tag coimmunoprecipitation physical, physical association 19615732 , 22426999 , 24145035 , 27463890 , 27880917 , 28514442 , (Europe PMC )0.40 BioGRID, IntAct USP12 Affinity Capture-MS, Affinity Capture-Western, Proximity Label-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 24145035 , 25216524 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct USP46 Affinity Capture-MS, Affinity Capture-Western, Biochemical Activity, Proximity Label-MS, Reconstituted Complex, anti tag coimmunoprecipitation physical, physical association 19615732 , 22391563 , 24145035 , 26186194 , 27880917 , 28514442 , (Europe PMC )0.40 BioGRID, IntAct WDR20 Affinity Capture-MS, Affinity Capture-Western, Proximity Label-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 25216524 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct WDR48 Affinity Capture-MS, Affinity Capture-Western, Proximity Label-MS, Reconstituted Complex, anti tag coimmunoprecipitation physical, physical association 19615732 , 24145035 , 25216524 , 27463890 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source CDH1 fluorescence microscopy colocalization 21701506 , (Europe PMC )0.27 IntAct, MINT DMWD Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , (Europe PMC )0.40 BioGRID, IntAct FKBP5 Affinity Capture-MS, Affinity Capture-Western, anti tag coimmunoprecipitation physical, physical association 19615732 , 27880917 , 28363942 , (Europe PMC )0.40 BioGRID, IntAct HDAC1 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct KSR1 anti tag coimmunoprecipitation association 27086506 , (Europe PMC )0.35 IntAct MAPK8 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct MARK2 anti tag coimmunoprecipitation physical association 19615732 , (Europe PMC )0.40 IntAct NEFL two hybrid physical association 23455924 , (Europe PMC )0.37 IntAct, MINT PHLPP2 Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct PPP5C Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , (Europe PMC )0.40 BioGRID, IntAct PRKCB anti tag coimmunoprecipitation, cosedimentation, phosphatase assay dephosphorylation reaction, physical association 18162466 , (Europe PMC )0.60 IntAct, MINT PTEN filter binding physical association 21804599 , (Europe PMC )0.40 IntAct RBM15 Affinity Capture-MS, Proximity Label-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct RPS6KB1 anti bait coimmunoprecipitation association 21986499 , (Europe PMC )0.35 IntAct SCRIB Affinity Capture-MS, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation physical, physical association 19615732 , 21701506 , 24145035 , (Europe PMC )0.59 BioGRID, IntAct, MINT SLC9A3R1 Affinity Capture-Western, Far Western, Reconstituted Complex, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, filter binding, fluorescence microscopy, pull down colocalization, direct interaction, physical, physical association 21804599 , (Europe PMC )0.52, 0.62 BioGRID, IntAct SLC9A3R2 Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct SNX27 Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , (Europe PMC )0.40 BioGRID, IntAct SUGT1 anti tag coimmunoprecipitation physical association 19615732 , (Europe PMC )0.40 IntAct USP1 Affinity Capture-MS, Affinity Capture-Western, Proximity Label-MS, Reconstituted Complex, anti tag coimmunoprecipitation physical, physical association 19615732 , 22426999 , 24145035 , 27463890 , 27880917 , 28514442 , (Europe PMC )0.40 BioGRID, IntAct USP12 Affinity Capture-MS, Affinity Capture-Western, Proximity Label-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 24145035 , 25216524 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct USP46 Affinity Capture-MS, Affinity Capture-Western, Biochemical Activity, Proximity Label-MS, Reconstituted Complex, anti tag coimmunoprecipitation physical, physical association 19615732 , 22391563 , 24145035 , 26186194 , 27880917 , 28514442 , (Europe PMC )0.40 BioGRID, IntAct WDR20 Affinity Capture-MS, Affinity Capture-Western, Proximity Label-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 25216524 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct WDR48 Affinity Capture-MS, Affinity Capture-Western, Proximity Label-MS, Reconstituted Complex, anti tag coimmunoprecipitation physical, physical association 19615732 , 24145035 , 25216524 , 27463890 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source NEFL two hybrid physical association 23455924 , (Europe PMC )0.37 IntAct, MINT PRKCB anti tag coimmunoprecipitation, cosedimentation, phosphatase assay dephosphorylation reaction, physical association 18162466 , (Europe PMC )0.60 IntAct, MINT SCRIB Affinity Capture-MS, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation physical, physical association 19615732 , 21701506 , 24145035 , (Europe PMC )0.59 BioGRID, IntAct, MINT
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source AIP Proximity Label-MS physical 27880917 , (Europe PMC )NA BioGRID AKT1 Affinity Capture-Western, Biochemical Activity physical 15808505 , 20605778 , 27097374 , (Europe PMC )NA BioGRID BAG2 Affinity Capture-MS physical 27880917 , (Europe PMC )NA BioGRID BRAP Reconstituted Complex, Two-hybrid physical 25820252 , (Europe PMC )NA BioGRID BRAP anti tag coimmunoprecipitation, confocal microscopy, two hybrid colocalization, physical association 25820252 , (Europe PMC )0.40, 0.44 IntAct BTRC Affinity Capture-Western physical 19797085 , 23440515 , (Europe PMC )NA BioGRID BUD23 Co-fractionation physical 22939629 , (Europe PMC )NA BioGRID CDH1 fluorescence microscopy colocalization 21701506 , (Europe PMC )0.27 IntAct, MINT COA1 Affinity Capture-MS physical 24145035 , (Europe PMC )NA BioGRID CSNK1A1 Biochemical Activity physical 19797085 , (Europe PMC )NA BioGRID DMWD Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , (Europe PMC )0.40 BioGRID, IntAct EFNB2 Affinity Capture-MS physical 26186194 , 28514442 , (Europe PMC )NA BioGRID ELAVL1 Affinity Capture-RNA physical 19322201 , (Europe PMC )NA BioGRID FANCI Affinity Capture-Western physical 27097374 , (Europe PMC )NA BioGRID FKBP4 Affinity Capture-MS physical 27880917 , (Europe PMC )NA BioGRID FKBP5 Affinity Capture-MS, Affinity Capture-Western, anti tag coimmunoprecipitation physical, physical association 19615732 , 27880917 , 28363942 , (Europe PMC )0.40 BioGRID, IntAct GLMN Proximity Label-MS physical 27880917 , (Europe PMC )NA BioGRID GSK3B Biochemical Activity physical 19797085 , (Europe PMC )NA BioGRID HAVCR2 Affinity Capture-MS physical 28514442 , (Europe PMC )NA BioGRID HDAC1 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct IRS4 Affinity Capture-MS physical 24145035 , (Europe PMC )NA BioGRID KSR1 anti tag coimmunoprecipitation association 27086506 , (Europe PMC )0.35 IntAct MAPK8 Affinity Capture-MS, anti tag coimmunoprecipitation association, physical 26496610 , (Europe PMC )0.35 BioGRID, IntAct MARK2 Affinity Capture-MS physical 19615732 , (Europe PMC )NA BioGRID MARK2 anti tag coimmunoprecipitation physical association 19615732 , (Europe PMC )0.40 IntAct MCMBP Affinity Capture-MS physical 27880917 , (Europe PMC )NA BioGRID NEFL two hybrid physical association 23455924 , (Europe PMC )0.37 IntAct, MINT NUDC Proximity Label-MS physical 27880917 , (Europe PMC )NA BioGRID NUDCD3 Affinity Capture-MS, Proximity Label-MS physical 27880917 , (Europe PMC )NA BioGRID PHLPP2 Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct PPP2R1A Affinity Capture-MS physical 27880917 , (Europe PMC )NA BioGRID PPP2R2A Affinity Capture-MS physical 27880917 , (Europe PMC )NA BioGRID PPP5C Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , (Europe PMC )0.40 BioGRID, IntAct PRKCB anti tag coimmunoprecipitation, cosedimentation, phosphatase assay dephosphorylation reaction, physical association 18162466 , (Europe PMC )0.60 IntAct, MINT PTEN filter binding physical association 21804599 , (Europe PMC )0.40 IntAct RBM15 Affinity Capture-MS, Proximity Label-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct RFPL2 Affinity Capture-MS physical 26186194 , 28514442 , (Europe PMC )NA BioGRID RPAP3 Affinity Capture-MS physical 27880917 , (Europe PMC )NA BioGRID RPS6KB1 anti bait coimmunoprecipitation association 21986499 , (Europe PMC )0.35 IntAct SCRIB Affinity Capture-MS, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation physical, physical association 19615732 , 21701506 , 24145035 , (Europe PMC )0.59 BioGRID, IntAct, MINT SKP2 Affinity Capture-MS physical 28514442 , (Europe PMC )NA BioGRID SLC9A3R1 Affinity Capture-Western, Far Western, Reconstituted Complex, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, filter binding, fluorescence microscopy, pull down colocalization, direct interaction, physical, physical association 21804599 , (Europe PMC )0.52, 0.62 BioGRID, IntAct SLC9A3R2 Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct SNX27 Affinity Capture-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , (Europe PMC )0.40 BioGRID, IntAct SNX6 Co-fractionation physical 22939629 , (Europe PMC )NA BioGRID STUB1 Affinity Capture-MS physical 24145035 , (Europe PMC )NA BioGRID SUGT1 Affinity Capture-MS, Affinity Capture-Western, Proximity Label-MS physical 19615732 , 23440515 , 24145035 , 27880917 , (Europe PMC )NA BioGRID SUGT1 anti tag coimmunoprecipitation physical association 19615732 , (Europe PMC )0.40 IntAct TRIM25 Affinity Capture-RNA physical 29117863 , (Europe PMC )NA BioGRID USP1 Affinity Capture-MS, Affinity Capture-Western, Proximity Label-MS, Reconstituted Complex, anti tag coimmunoprecipitation physical, physical association 19615732 , 22426999 , 24145035 , 27463890 , 27880917 , 28514442 , (Europe PMC )0.40 BioGRID, IntAct USP12 Affinity Capture-MS, Affinity Capture-Western, Proximity Label-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 24145035 , 25216524 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct USP46 Affinity Capture-MS, Affinity Capture-Western, Biochemical Activity, Proximity Label-MS, Reconstituted Complex, anti tag coimmunoprecipitation physical, physical association 19615732 , 22391563 , 24145035 , 26186194 , 27880917 , 28514442 , (Europe PMC )0.40 BioGRID, IntAct WDR20 Affinity Capture-MS, Affinity Capture-Western, Proximity Label-MS, anti tag coimmunoprecipitation physical, physical association 19615732 , 25216524 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct WDR48 Affinity Capture-MS, Affinity Capture-Western, Proximity Label-MS, Reconstituted Complex, anti tag coimmunoprecipitation physical, physical association 19615732 , 24145035 , 25216524 , 27463890 , 27880917 , (Europe PMC )0.40 BioGRID, IntAct
Phosphorylating Kinases Phosphoresidues Detection Method PubMed IDs Source
Visualize a network of protein kinases and phosphatases phosphorylating and dephosphorylating this protein respectively
CSNK1A1 S1359_VPRPHVQsVLLTPQD , S1379_GSKGLWDsLSVEEAV , S1381_KGLWDSLsVEEAVEA , T1363_HVQSVLLtPQDEFFI , NA NA PhosphoSitePlus , GSK3B S1359_VPRPHVQsVLLTPQD , S1379_GSKGLWDsLSVEEAV , S1381_KGLWDSLsVEEAVEA , T1363_HVQSVLLtPQDEFFI , NA NA PhosphoSitePlus ,
Statistics
